中检院中青年基金项目(2013NC3)。
目的 分析比较BALB/c和CBA小鼠局部淋巴结试验结果。方法 将已知致敏性的4种化学物(2,4-二硝基氯苯、丁子香酚、己基肉桂醛和异丙醇)和未知致敏性的3种化妆品对BALB/c和CBA小鼠连续染毒3 d, 第5天腹腔注射示踪剂BrdU, 第6天分离颌下淋巴结制取单细胞悬液, 用ELISA试盒检测细胞增殖。结果 2,4-二硝基氯苯、丁子香酚、己基肉桂醛和3号烫发剂两品系结果均呈致敏阳性, 3种化学物EC1.6值分别为0.08%(极强)、4.02%(中)、6.68%(中)(BALB/c)和0.07%(极强)、6.08%(中)、8.89%(中)(CBA);异丙醇、1和2号化妆品两品系结果均为致敏阴性, SI均小于1.6。结论 两品系小鼠局部淋巴结试验结果较一致, BALB/c可替代CBA小鼠应用于化学物和化妆品的致敏性评价。
Objective To analyze and compare results of local lymph node assays (LLNA) between BALB/c and CBA mice. Methods 4 chemicals (2, 4-dinitrochlorobenzene, eugenol, hexyl cinnamic aldehyde and isopropanol) and 3 cosmetics were applied to the dorsum of both ears of Balb/c and CBA mice for three consecutive days. BrdU solution was injected inter-peritoneally on day 5. On day 6, the bilateral draining auricular lymph nodes were excised and made into single cell suspension, the lymph cell proliferation was measured by BrdU ELISA kit. Results 2, 4-dinitrochlorobenzene, eugenol, hexyl cinnamic aldehyde and NO.3 perm agent pretended positive for both strains, EC1.6 values of three chemicals were found to be 0.08% (very strong), 4.02% (moderate), 6.68% (moderate) and 0.07% (very strong), 6.08% (moderate), 8.89% (moderate) for BALB/c and CBA mice respectively. Isopropanol, NO.1 and NO.2 cosmetics pretended to be non-sensitizers with SI<1.6 for both strains. Conclusion This study showed that BALB/c mouse was essentially equal to CBA for LLNA: BrdU-ELISA, which suggested that BALB/c mouse was a good alternative for CBA used in chemicals and cosmetics allergenic evaluation.
胡培丽,张露勇,李波,邢书霞.两品系小鼠局部淋巴结试验结果比较[J].中国比较医学杂志,2015,25(5):54~57.
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