Objective To explore effects of parents exposure to TBT on blood routine of F1 generation mice. Methods 80 mice including 40 males and 40 females, were randomly divided into control groups (CK), low dose groups (LTBT), middle dose groups (MTBT) and high dose groups (HTBT). They were given dose of TBT (0, 0.2, 2, 20μg/kg) every day. The experiment lasted 45 days. At 60 days, one female and one male of the same concentration were bred in the same cage according to 1:1. At postnatal day 60, blood was collected for the determination of blood routine. Results Compared with control group, the number of red blood cells and hemoglobin of F1 generation male mice in LTBT and HTBT groups were significantly increased (P < 0.01); Red blood cell volume, mean corpuscular hemoglobin (P < 0.01), and the lymphocyte absolute value in F1 generation male LTBT were significantly reduced (P < 0.05); HTBT of female mice were significantly increased about the number of red blood cells (P < 0.01). A dose-dependent increase of the hemoglobin, red blood cells, and platelet count of F1 generation female experimental groups was observed. Conclusion Parental TBT exposure affects the F1 mice blood routine. There is the greatest influence on LTBT in F1 generation male mice and on HTBT in F1 generation female mice.