三种不同严重程度大鼠重症胰腺炎模型比较研究
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国家自然科学基金青年基金(81302929);上海市教委科研创新项目(14YZ065);上海市杏林新星计划(ZYSNXD011-RC-XLXX-20130019)。


Comparative study of severe acute pancreatitis model in rats with three different severity
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    摘要:

    目的 为研究者选择合适的牛磺胆酸钠浓度来制备大鼠重症胰腺炎(severe acute pancreatitis,SAP)SAP模型提供依据。方法 将60只SD大鼠随机分为假手术组、1.5%浓度组、3.5%浓度组和5%浓度组, 造模各组分别用1.5%、3.5%和5%牛磺胆酸钠按逆行胆胰管注射法制备SAP模型。术后统计各组大鼠的死亡率;检测血清淀粉酶、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平;观测各组大鼠胰腺组织HE染色病理评分。结果 5%浓度组死亡率较1.5%浓度组显著升高, 血淀粉酶、肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平、出血和腺泡组织坏死的病理评分较1.5%浓度组和3.5%浓度组均有显著升高。结论 5%的牛磺胆酸钠逆行胆胰管注射法能更好的制备SAP模型, 且更符合SAP的生理、病理表现。

    Abstract:

    Objective to provide the evidence for inducing the SAP model in rats with proper concentration of sodium taurocholate. Methods 60 SD rats were divided into sham operated group, group of 1.5% in concentration, group of 3.5% in concentration and group of 5% in concentration randomly, while the SAP model was induced by the sodium taurocholate concentration of 1.5%, 3.5% and 5% with the method of retrograde injection into the biliopancreatic duct.To calculate the mortality of different groups, measure the serum amylase, tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6), and to observe the pancreatic pathological scores of HE staining in rats. Results The mortality in group of 5% in concentration has a significant ascending compared with group of 1.5% in concentration, while the serum amylase, tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), pathological score of hemorrhage and acinar necrosis in group of 5% in concentration have a significant ascending compared with group of 1.5% in concentration and group of 3.5% in concentration. Conclusions A better SAP model may be induced by sodium taurocholate with the concentration of 5% by the method of retrograde injection into the biliopancreatic duct, which may accord with the physiological and pathological manifestation of SAP.

    参考文献
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    [8] 余夙慧, 康牧星, 孙洪伟, 等.SIRS发展在胰腺坏死、继发感染致胰腺炎病变加重中的作用[J].肝胆外科杂志, 2014, 26(1):43-47.
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袁琳,周秉舵,王晓素,吴中华,孔婧,徐亭亭,王宏伟,朱生樑,方盛泉,周莉,刘春芳,张秀莲,王轶.三种不同严重程度大鼠重症胰腺炎模型比较研究[J].中国比较医学杂志,2015,25(5):20~23.

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