探讨姜黄素和茶多酚对不同时间UVB致无毛鼠急性光损伤的防御作用
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2013年广州市医药卫生科技项目(20131A011131);2013年广东省科技计划项目(2013B021800044);2014年广东省医学科研基金(A2014592);"十二五"专项课题(2011ZXJ09201-031)。


Protective effect of Curcumin and Tea Polyphenols against different time of UVB-induced acute photodamage in hairless mice skin
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    摘要:

    目的 探讨姜黄素和茶多酚对不同时间UVB致无毛鼠急性光损伤的防御作用。方法 取清洁级无毛鼠36只, 随机分为对照组及药物组(0 s、30 s、60 s、120 s、240 s);每组各2只。于照射前30 min在无毛鼠背部涂抹姜黄素或茶多酚, 照射距离为15 cm, 采用3倍最小红斑量(MED)的UVB约200~540 mJ/cm2分别照射各组, 取皮肤组织, 制成石蜡切片, 光学显微镜观察。结果 对照组无毛鼠皮肤出现不同程度鳞屑, 胶原间可见散在淋巴细胞, 且120 s及240 s时出现胶原红染、排列紊乱、均质化等改变。药物组无毛鼠真皮中炎性细胞浸润减少, 胶原受损现象得到改善。结论 外用姜黄素和茶多酚可通过减轻炎症细胞浸润及减轻胶原受损程度而防御UVB致无毛鼠皮肤急性光损伤。

    Abstract:

    Objective To assess the protective of Curcumin and Tea Polyphenols against different time of UVB-induced acute photo damage in hairless mice skin. Methods Thirty six BALB/C hair1ess mice were randomly divided into control groups and treatment groups(all of them were divided into 0 s, 30 s, 60 s, 120 s, 240 s groups with two hairless mice).Curcumin and Tea Polyphenols were applied to the mice’s skin before 30min irradiation. The irradiation distance was 15cm. The mice in each group were given three times of UVB MED about 200~540 mJ/cm2 and the irradiation time were 30 s、60 s、120 s、240 s. After irradiation, the skin on the back of the radiation center were taken into paraffin section for light microscopy observation. Results Different amount of squamae appeared in the control group of hairless rat, and lymphocytes scattered in collagen tissues. In the 120 s and 240 s UVB irradiated group, the collagen was red stained, disorderly arranged, even homogenized. In the treatment group, inflammatory cell infiltration was lessened, and collagen damage weakened. Conclusion By weakening inflammatory cell infiltration and collagen damage, topical application of Curcumin and Tea Polyphenols can protect hairless mice from acute photodamage caused by UVB irradiation.

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高爱莉,江娜,刘清,张倩雯,范君文,王鹏,邓蕙妍,田歆,梁碧华,李润祥,马少吟,李振洁,龚业青,朱慧兰.探讨姜黄素和茶多酚对不同时间UVB致无毛鼠急性光损伤的防御作用[J].中国比较医学杂志,2015,25(5):9~12.

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  • 最后修改日期:2015-04-03
  • 在线发布日期: 2015-05-29
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