国家科技支撑计划课题《实验动物质量监测及规范管理体系建设与完善(编号:2013BAK11B03)》。
目的 对国家啮齿类实验动物种子中心上海分中心KM小鼠种子群体进行群体遗传质量分析与评价。方法 利用筛选出的30个微卫星位点设计引物,对种群中随机抽取的30只KM小鼠样本进行PCR扩增,扩增产物利用STR扫描技术进行测定,用Popgen1.32软件对所得结果进行处理分析。结果 KM小鼠种子群体共得到123个等位基因型,平均有效等位基因数为2.3989,平均杂合度为0.5342,平均多态性信息含量(PIC)为0.4735。结论 上海分中心KM小鼠种子群体具有良好的遗传稳定性和多样性,符合封闭群动物的群体遗传概貌特征。
Objective To analyze and evaluate the population genetic quality of outbred KM mice from National Rodent Seed Center (Shanghai). Methods A total of 30 outbred KM mice were randomly chosen. The genetic characteristics of the population were determined by PCR and STR scanning using 30 selected microsatellite loci. Popgen1.32 software was used to process the data.Results Thirty microsatellite loci shared 123 alleles in the KM mouse population. The average effective allele number and the average heterozygosity were 2.3989 and 0.5342, respectively. The average polymorphism information content (PIC) was 0.4735. Conclusions The outbred KM mouse population of Shanghai Seed Center has genetic stability and genetic diversity, and is satisfied with the genetic characteristics of closed colony laboratory animal.
王洪,杜小燕,徐平,迟晓丽,李根平,岳秉飞,陈振文.上海KM小鼠种子群体遗传状况分析[J].中国比较医学杂志,2014,24(12):27~32.
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