Objective To provide an experimental reference for clinic application of Jiazhu vascular ligating clips on ligation effects and the impact on the body in rabbits after laparoscopoic cholecystectomy. Methods 36 rabbits were randomly divided into 3 groups, normal control group, Jiazhu group and similar product control (Hem-o-lok) group, 12 rabbits in each group. Except for normal control group, the rabbits were used gallbladder duct and gallbladder artery closed by vascular ligating clips and Hem-o-lok clips in Jiazhu group and similar product control group, respectively. The changes of blood biochemical, electrolytes, hematological parameters, coagulation, ligation effects and organ coefficients were observed at post-operation during 12 months. Results Compared with normal control group, the rabbit weight was reduced in the Jiazhu group at 3 days postoperatively (P< 0.01), ALT level was increased at 1week postoperatively (P< 0.05), CREA and ALB levels were all significant decreased at 1～2 weeks postoperatively (P< 0.05, P< 0.01), NEUT numbers and TG content were significant increased at 2 weeks postoperatively (P< 0.05), GLU content was significant decreased at 2 weeks and 1 month postoperatively (P< 0.01); LYM and RBC numbers were significant decreased at 1 month and 1 week postoperatively (P< 0.05), respectively. PLT numbers were significant increased at 1～2 weeks postoperatively (P<0 .01). While the rabbit weight was reduced in the Hem-o-lok group between 3 days and 1month postoperatively (P< 0.05, P< 0.01), and ALT level, NEUT and PLT numbers were increased at 1 week postoperatively (P< 0.05, P< 0.01), ALB and GLU contents were all significant decreased at 2 weeks postoperatively (P< 0.01), TC content was significant increased at 1～2 weeks postoperatively (P< 0.05). The rabbit weight, blood biochemical and hematological parameters in the Jiazhu group and Hem-o-lok group were all no significant differences with normal control group in the remaining time (P> 0.05), and there were all no significant differences with electrolyte, coagulation and organ coefficients between each group (P> 0.05). Conclusion Jiazhu vascular ligating clips are safe and reliable to close gallbladder duct and blood vessels, It shows that there are no differences in the validity and security between Jiazhu vascular ligating clips and Hem-o-lok clips.