国家科技支撑课题（2013BAK11B01）。
目的 肿瘤移植裸鼠粪类圆虫病诊断。方法 肿瘤移植裸鼠的尸体解剖显微镜检查发现类圆线虫作形态学鉴定并采用二重PCR进行分子诊断。 结果 尸检发现肿瘤移植裸鼠样本中出现大量粪类圆线虫，初步确诊粪类圆线虫病，二重PCR检出样本中粪类圆线虫特定DNA进一步确诊。结论 防止这种严重后果是早期诊断。肿瘤移植受体和供体应进行寄生虫感染包括类圆线虫病筛查。本研究第一个广泛报道了肿瘤移植裸鼠粪类圆虫病诊断。
Objective To diagnosis tumor transplanted nude mice Strongyloidiasis. Methods Postmortem microscopic examination of the tumor transplanted nude mice detected Strongyloides stercoralis for morphological identification and double polymerase chain reaction (PCR) assay for molecular diagnosis of Strongyloides stercoralis infection in tumor transplanted nude mice. Results Presence of numerous S. stercoralis in autopsy in tumor transplanted nude mice samples preliminary determined the diagnosis of strongyloidiasis. Confirmed diagnosis of Strongyloides stercoralis infection by double PCR detection of specific DNA in tumor transplanted nude mice samples. Conclusion The most important clue to prevent such serious consequences is early diagnosis. Tumor transplanted recipients and donors should be screened for parasitic infections including strongyloidiasis. To the authors' knowledge, this study is the first extensive report on diagnosis tumor transplanted nude mice Strongyloidiasis.
高正琴,贺争鸣,关伟鸿.肿瘤移植裸鼠粪类圆线虫感染病原和分子诊断[J].中国比较医学杂志,2014,24(7):51~53,6.
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