Objective To measure the circadian rhythm of consecutive monitoring electrocardiogram(ECG), blood pressure, respiration, activities in Bama minipigs by telemetry technology. Methods Six 6-month-old Bama minipigs were implanted with VAP port in Superficial femoral artery, after recovery 7 days, 24 hours ECG, blood pressure, activity and respiratory parameters were recorded and analyzed in the EMAK noninvasive telemetry system. Results The ECG, blood pressure, respiration and activities in Bama minipigs showed circadian rhythm changes, Bama minipigs daytime heart rate was significantly higher than the nighttime heart rate (P< 0.01), and daytime PR interval, QRS interval and QT interval was significantly lower than the nighttime (P< 0.05, P< 0.01), daytime mean heart rate was 76.22 beats/min, nighttime mean heart rate was 67.03 night beats/min; daytime mean PR interval, QRS interval and QT interval were 109.97 ms, 42.72 ms and 380.37 ms, nighttime mean PR interval, QRS interval and QT interval were 112.32ms, 44.01ms and 389.24ms. Bama minipigs daytime systolic blood pressure, diastolic blood pressure and mean blood pressure were significantly higher than the nighttime (P< 0.01), daytime mean systolic blood pressure, diastolic blood pressure and mean blood pressure were 129.57 mmHg, 96.75 mmHg and 111.73 mmHg, nighttime mean systolic blood pressure, diastolic blood pressure, mean blood pressure were 122.81 mmHg, 92.65 mmHg and 106.19 mmHg, and the nighttime reduction rate of systolic blood pressure, diastolic blood pressure and mean blood pressure were 19.89%, 19.05% and 19.35%. In addition, Bama minipigs daytime activity and respiratory rate were significantly higher than the nighttime (P< 0.01). Conclusion It's Feasible to measure the circadian rhythm of electrocardiogram(ECG), blood pressure, activity and respiratory parameters in Bama minipigs by telemetry technology, and can truly shows those physiological parameters of the Bama minipigs within 24h, to provide a reference for pharmacology and toxicology research by using Bama minipigs.