利用小动物SPECT/CT和<sup>99m</sup>锝-葡糖二酸评估急性缺血坏死心肌动物

doi:10.3969.j.issn.1671.7856. 2014.007.003

首页 > 过刊浏览>2014年第24卷第7期 >14-18. DOI:10.3969.j.issn.1671.7856. 2014.007.003

利用小动物SPECT/CT和99m锝-葡糖二酸评估急性缺血坏死心肌动物
DOI:
                        10.3969.j.issn.1671.7856. 2014.007.003
                    
作者:
                        宝璐尔宝璐尔
同济大学附属东方医院心脏外科, 上海 200120
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张林张林
同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120
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周晓慧周晓慧
同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120
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孟庆姝孟庆姝
同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120
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占贞贞占贞贞
同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120
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范慧敏范慧敏
同济大学附属东方医院心脏外科, 上海 200120;同济大学附属东方医院心衰专科, 上海 200120;同济大学附属东方医院心衰研究所, 上海 200120
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基金项目:国家自然科学基金（81070073，81273263）；上海市医学重点专科（ZK2012A27）；上海领军人才（2012-053）；上海市浦东新区卫生系统领先人才培养计划资助（PKR2011-01）。

In vivo imaging of ^99mTc-Glucarate-labeled acute necrotic myocardium using microSPECT/CT

Author:

BAO Lu-er
BAO Lu-er
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China
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ZHANG Lin
ZHANG Lin
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
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ZHOU Xiao-hui
ZHOU Xiao-hui
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
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MENG Qing-shu
MENG Qing-shu
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
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ZHAN Zhen-zhen
ZHAN Zhen-zhen
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
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FAN Hui-min
FAN Hui-min
Department of Cardiac Surgery, Shanghai East Hospital, TongJi University, Shanghai 200120, China;Heart Failure department, Shanghai East Hospital, Tongji University, Shanghai 200120, China;Heart Failure Institute, Shanghai East Hospital, Tongji University, Shanghai 200120, China
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摘要:

目的利用^99m锝-葡糖二酸（^99mTc-Glucarate）和小动物SPECT/CT评估大鼠急性心肌缺血再灌注损伤后缺血坏死心肌的位置和范围。方法建立大鼠急性缺血再灌注损伤模型，手术1 d后尾静脉注射^99mTc-Glucarate，注射30 min后利用小动物SPECT/CT融合技术分析^99m锝-葡糖二酸标记的心肌组织的位置和范围，并与氯化三苯基四氮唑（triphenyltetrazolium chloride，TTC）染色法标记的缺血坏死心肌比较。结果小动物SPECT/CT结果显示手术组心肌坏死部位的^99m锝-葡糖二酸的放射性摄取率（心肝比值 1.90±0.33）明显高于正常组大鼠（P< 0.05），利用小动物SPECT/CT融合技术定位的缺血坏死心肌范围和TTC染色法的测量结果呈线性相关（R²=0.964）。结论通过^99m锝-葡糖二酸可以特异性地标记急性缺血坏死心肌，利用小动物SPECT/CT融合技术可以无创性地分析急性缺血坏死心肌的位置和范围。

关键词:大鼠;缺血/再灌注损伤;^99m锝;葡糖二酸;SPECT/CT

Abstract:

Objective To evaluate the anatomic localization and size of acute necrotic myocardium in the ischemic-reperfused rat hearts using ^99mTC-Glucarate and microSPECT/CT. Methods The ischemic-reperfused (IR) rat heart models were established by ligating left anterior descending coronary artery for 60 min. ^99mTC-Glucarate was intravenously injected into the rats 24 hours after IR operations. Images were acquired 30 min after administration of ^99mTC-Glucarate using microSPECT/CT. Anatomic localization and size of acute necrotic myocardium were analyzed with microSPECT/CT imaging,and these results were compared to those determined by triphenyltetrazolium chloride (TTC) staining. Results The microSPECT/CT images showed hot spot accumulations of ^99mTC-Glucarate in IR hearts (the heart-to-liver ratio was 1.90±0.33), not in controls (P < 0.05). The anatomic localization of ^99mTC-Glucarate-labeled necrotic myocardium were in correspondence with TTC staining results. The hot spot size was related significantly to necrotic myocardial size determined by TTC staining (R²=0.964). Conclusions The localization and size of acute necrotic myocardium can be assessed by non-invasive microSPECT/CT imaging with^99mTc-Glucarate.

Key words:Rats;Ischemic-reperfusion injury;^99mTc;Glucarate;microSPECT/CT

参考文献

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宝璐尔,张林,周晓慧,孟庆姝,占贞贞,范慧敏.利用小动物SPECT/CT和^99m锝-葡糖二酸评估急性缺血坏死心肌动物[J].中国比较医学杂志,2014,24(7):14~18.

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最后修改日期:2014-05-20
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在线发布日期: 2014-08-19
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