Objective To establish the CTRP4 transgenic mouse model and investigate the function of the novel adipocytokine CTRP4. Methods CTRP4 overexpressing vector in pCAGGS was firstly constructed and then microinjected into zygote to establish the founder transgenic mice. F1 heterozygotes were generated by founder mice mating with wildtype mice, and the CTRP4 transgenic homozygotes were generated by F1 littermates. The genotype was confirmed by PCR and test cross method. The expression level of CTRP4 in transgenic mice was detected by western blot. Result The human CTRP4 transgenic homozygote mice line was established, and the expression level of CTRP4 was confirmed raletively high in detected tissues including heart, liver, brain and kidney. Conclusion The human CTRP4 transgenic mice was successfully established.