Objective To explore the correlation of internal environment with estradiol-induced male SD rat models of chronic prostatitis，and provide an experimental approach for its pathogenesis and treatment researches. Methods Anesthesia with 3% pentobarbital, castration surgery under the sterile condition, and recovery for five days were performed in the experiment. Thirty male SD rats were randomly divided into 3 groups, 10 rats in each group. 0.25mg/kg estradiol (low dose) and 1.25 /kg estradiol (high dose), respectively, were subcutaneously injected from day 6 for 30 consecutive days. The solvent control group received injection of olive oil in the same period. Results WBC, RBC, HGB and HCT were significantly lower in the estrogen groups than that in the control group (P<0.01). PAP was significantly decreased (P<0.01). Testosterone was significantly increased in the high-dose group (P<0.01), and C-reactive protein (CRP) also showed an increasing trend in the estrogen groups, but statistically not significant. There was no significant difference in the gross appearance of the prostate among the groups. Histopathological analysis revealed that there were inflammatory infiltration and accumulation of brown particles in the spleen tissue in both the low dose and high dose estrogen groups. There were no significant changes in other organs. Organ and organ coefficiente of the thymus and spleen were significantly reduced. Conclusions Our findings indicate that chronic non-bacterial prostatitis induced by estrogen can lead to a series of changes of the internal environment including the hematology, serum biochemistry, hormone levels, organs and organ coefficient.