季节性流感疫苗免疫血清与H7N9禽流感病毒交叉反应
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基金项目:

十二五科技重大专项(2012ZX10004501-004-003,2012ZX10004501-004-004,2012ZX10004301-8);国家自然科学基金(31370203);科技部H7N9禽流感应急防控专项(KJYJ-2013-01-04)。


Analysis of cross-protective immunity of neutralizing antibodies to H7N9 influenza virus raised in sera vaccinated with seasonal influenza vaccine
Author:
  • LV Qi

    LV Qi

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • BAO Lin-lin

    BAO Lin-lin

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • XU Li-li

    XU Li-li

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • DENG Wei

    DENG Wei

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • LI Feng-di

    LI Feng-di

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • YUAN Jing

    YUAN Jing

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • YU Pin

    YU Pin

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • YAO Yan-feng

    YAO Yan-feng

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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  • QIN Chuan

    QIN Chuan

    Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Beijing Union Medicine College, Beijing 100021, China
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    摘要:

    目的 分析接种季节性流感疫苗人群是否具有对新发H7N9流感病毒具有交叉保护,并反向检测了雪貂产生H7N9抗血清与H1/H3/H5不同亚型流感病毒的免疫反应。方法 利用血凝抑制试验和微量中和实验检测疫苗免疫后人群转阳血清对A/Anhui/1(H7N9)病毒中和作用及雪貂H7N9抗血清与A/California/07/2009(H1N1)、A/PR/8/34(H1N1)、A/Brisbane/59/2007(H1N1)、A/Brisbane/10/2007(H3N2)、A/Shenzhen/406H/2006(H5N1)和A/Vietnam/1203/2004(H5N1)等不同亚型病毒的中和作用。结果 季节性流感疫苗受众者转阳血清与H7N9病毒反应HI及中和抗体反应均为阴性,雪貂H7N9抗血清与H1/H3/H5不同亚型流感病毒反应HI及中和抗体反应均为阴性。结论 季节性流感疫苗接种人群不具有对新发H7N9流感病毒交叉保护,并且反向验证了H7N9病毒抗血清与 H1/H3/H5亚型流感病毒亦无交叉免疫反应。

    Abstract:

    Objective To investigate whether seasonal vaccine induces anti-virus activity against H7N9 and cross-reactive immune response against related H1/H3/H5subtype influenza viruses.Methods A/Anhui/1 (H7N9) were pre-incubated with sera vaccinated with seasonal vaccine and A/California/07/2009 (H1N1), A/PR/8/34 (H1N1), A/Brisbane/59/2007 (H1N1), A/Brisbane/10/2007 (H3N2), A/Shenzhen/406H/2006 (H5N1), A/Vietnam/1203/2004 (H5N1) were pre incubated with ferret sera infect with H7N9 through Micro-neutralization and HI Assay. Results Human sera from the donors were tested for HI and neutralization activity against influenza (H7N9) preparationsremained at undetectable levels after seasonal vaccination, and ferret sera were tested for HI and neutralization activity against H1/H3/H5 type influenza viruses either.Conclusion The neutralizing antibodies raised in all donors sera vaccinated with seasonal vaccine cannot neutralize H7N9 influenza virus, and cannot cross-react to H1/H3/H5 subtype.

    参考文献
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吕琦,鲍琳琳,许黎黎,邓巍,李枫棣,袁静,于品,姚艳丰,秦川.季节性流感疫苗免疫血清与H7N9禽流感病毒交叉反应[J].中国比较医学杂志,2014,24(1):59~61.

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  • 最后修改日期:2013-11-08
  • 在线发布日期: 2014-01-23
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