十二五重大专项（2012ZX10004501-004-003，2012ZX10004501-004-004，2012ZX10004301-8）；国家自然科学基金（31370203）；科技部H7N9禽流感应急防控专项（KJYJ-2013-01-04）；协和青年教师培养项目（2012Y2，2012D15）。
目的 进一步探讨H7N9禽流感病毒对哺乳动物的感染与致病能力，并对其分子机制进行研究。方法 以病毒滴鼻感染小鼠，在BALB/c小鼠肺组织中连续传代，通过病毒全基因组测序及比对探寻适应的分子机制。结果 禽流感H7N9 A/Anhui/1/2013病毒，经过在小鼠体内进行9次传代后，对鼠肺适应株与野生型毒株进行基因比对，发现适应株HA基因，NA基因以及PA亚基共发生了4个有义突变。结论 禽流感H7N9 A/Anhui/1/2013病毒经连续传代后基因发生突变，但对病毒的毒力以及致病力影响仍需后续实验验证。
Objective To discuss the infective and pathogenic ability of H7N9 avian influenza virus in mammals.Methods The mice model was induced by nose dropping method with influenza virus, by continuous passage in the lung tissue of BALB/c, to get the adaption of seasonal H1N1 in mice and seasonal influenza virus infection model, the lung index and death rate was observed. Results After 9 times continuous passage in the lung tissue of BALB/c, Genome sequencing and alignment indicate that the HA gene, NA gene and PA gene was mutated.Conclusion H7N9 Avian influenza virus A/Anhui/1/2013 gene mutation occurs after continuous passage, but the virμLence and pathogeniceffects of the virus still need further verification.
李枫棣,鲍琳琳,朱华,邓巍,许黎黎,吕琦,秦川. H7N9禽流感病毒在小鼠体内的适应性[J].中国比较医学杂志,2014,24(1):56~58.
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