国家自然基金面上项目（81070926）。
目的 研究缺氧对原代培养星形胶质细胞DNA甲基化相关酶及组蛋白乙酰化相关酶水平的影响。方法 原代培养的星形胶质细胞经氧糖剥夺处理后，Western blot方法检测细胞DNA甲基转移酶，DNA去甲基转移酶，组蛋白乙酰化酶及组蛋白去乙酰化酶的表达。结果 原代培养的星形胶质细胞缺氧处理48 h后，DNA甲基转移酶的表达升高，DNMT3A和DNMT1与对照组相比分别升高了49%和83%，DNA去甲基转移酶的表达降低了36%；组蛋白去乙酰化酶的表达升高到对照组的2.1倍，而组蛋白乙酰化酶的表达则升高得更为明显，为对照组的20倍。结论 缺氧可以改变星形胶质细胞DNA甲基化和组蛋白乙酰化相关酶的表达，从而影响基因的表达。
Objective To investigate the role of hypoxia on the expression of DNA methylation and histone acetylation related enzymes.Methods Primary cultured rat astrocytes treated with oxygen-glucose deprivation (OGD) were used. The expressions of DNA methyltransferase (DNMTs), Methl-CpG binding domain protein 2(MBD2), CREB-binding protein (CBP), Histone Deacetylase 3(HDAC3) were examined by Western blot.Results DNMTs(DNMT3A and DNMT1) levels were increased by 49% and 83% compared with control and the level of MBD2 level was decreased by 36% after OGD for 48h. HDAC3 level was up-regulated by 2.1 times after OGD for 48h, however the expression of CBP was dramatically increased by 20 times compared with control.Conclusion The expression of DNA methylation and histone acetylation related enzymes might alter under hypoxia in astrocytes leading to gene transcription.
杨清麟,李良.缺氧对星形胶质细胞DNA甲基化及组蛋白乙酰化相关酶的表达影响[J].中国比较医学杂志,2014,24(1):26~30.
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