The biochip technology is used to build micro-biochemical analysis systems on the chip surface, through micro-processing and micro-electronics technology. It can make high-throughput detection of tissue cells, proteins, DNA or other biological components. Biochips are widely used for life sciences, judicial expertise, food and nutritional science, environmental science, agriculture and forestry science, military science and other areas. This article makes a brief summary of the application of biochip in cancer research, diagnosis and treatment.