管彤 (1968-),女,副研究员,从事卫生毒理学及实验发育生物学研究。
天津市自然科学重点基金项目资助 (编号:003803511)。
目的 建立野生来源TW (Tianjin wild, TW)近交系小鼠的体重和血液生化正常范围并检测其毛色基因纯合性。方法分别选用F23代TW近交系成年小鼠,进行毛色基因测试,并检测动物的体重及血液生化指标。结果6周龄前,雌雄TW小鼠体重差异无统计学意义(P<0.05)；6周龄后雄性TW小鼠体重明显高于同期雌性小鼠体重,差异具有统计学意义(P<0.05)。血生化检测指标中,雌雄小鼠的总蛋白和甘油三酯均值不同,差异具有统计学意义(P<0.05),其他各项差异均无统计学意义(P>0.05),且与文献报道的其他品系结果不一致。毛色基因测试,F1代小鼠的毛色为白腹野生色,其基因型为AWAWBBccDD。结论TW小鼠毛色基因已达纯合,且在一些指标上与通用实验小鼠品系不同,具有自身特点。
ObjectiveTo establish the normal range of body weight and blood biochemical indexes of Tianjin wild (TW) inbred mice, and to determine their coat color gene. MethodsThe body weight and blood biochemical parameters of TW inbred mice were examined. The coat color gene was determined by mating with DBA/2 mice. ResultsWhen the TW mice were younger than six weeks old, there was no significant difference between the body weights of TW male and female mice (P<0.05). When the TW mice were older than six weeks of age, the body weight of TW male mice was higher than that of TW female mice (P<0.05). Compared with the female group, the total protein was higher in male group (P<0.05), while the triglycerides were lower (P<0.05). The coat color gene analysis revealed that hair color of first generation hybrid was white belly wild color, and its gene type was AWAWBBccDD. ConclusionsTW inbred mice have their own characteristic blood biochemical indexes, and some are different from those of commonly used inbred mouse strains. The coat color gene of TW mice is homozygosis.
管彤,张静姝,李大鸣,于国德,王春花,王凤山. TW近交系小鼠的血液生化及毛色基因检测[J].中国比较医学杂志,2012,(4):39~42.
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