不同品系小鼠感染甲型流感病毒肺小血管内血栓形成
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刘颖(1981-),研究实习员,研究方向:病理与病理生理。

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中国医学科学院医学实验动物研究所基本科研业务费:DWS200904;北京市科委项目:Z09000700090904;中医药行业科研专项:200907001-5。


Microvascular Thrombosis in the Lung of Different Strains of Mice Infected with Influenza A Virus
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    摘要:

    目的本实验旨在观察不同品系小鼠感染甲型流感病毒后肺组织内血栓形成的情况。方法使用H1N1病毒A/California/7/2009(CA7)株和H3N2病毒A/Brisbane/10/07株,对BALB/C小鼠、Scid小鼠、NOD/LTJ小鼠、BALB/C-nu小鼠、NOD-Scid小鼠和icosl-KO小鼠经乙醚麻醉后进行滴鼻攻毒。检测小鼠感染后肺组织病毒拷贝数并观察肺组织病理学改变。结果H1N1和H3N2滴鼻攻毒的各组小鼠均染毒,病理表现为程度略有差异的间质性肺炎。13只H1N1病毒感染小鼠和6只H3N2感染小鼠在肺组织中观察到多个小血管内有血栓形成,血栓成分主要为纤维素和血小板。结论各品系小鼠感染H1N1和H3N2流感病毒后均可能出现肺组织内血栓形成。

    Abstract:

    ObjectiveTo investigate thrombosis in mouse lung tissue after infection with influenza A virus, and compare the differences between mouse strains with different immune status. MethodsInfluenza H1N1(A/California/7/2009) and H3N2(A/Brisbane/10/07) were used in this infectivity study. Forty BALB/c mice, 50 NOD-Scid mice, 33 Scid mice, 33 BALB/C-nu mice, 33 NOD/LTJ mice and 10 icosl-KO mice were anesthetized by ether inhalation and inoculated intranasally with 0.05 mL suspension of infectious virus H1N1 or H3N2. The pathogenic changes were observed and the virus copy numbers in the lung tissue were detected by real-time PCR. ResultsVarying degrees of interstitial pneumonia were observed in the lung tissue of mice infected with H1N1 and H3N2 viruses. There was thrombosis within the lung tissue in 13 mice of H1N1 group and 6 mice of H3N2 group. The main components of the thrombus were cellulose and platelets. ConclusionsOur results support that thrombosis may occur in the lungs of all studied mouse strains after infection with influenza A (H1N1) and (H3N2) viruses. 

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刘颖,徐艳峰,邓巍,朱华,黄澜,马春梅,鲍琳琳,秦川.不同品系小鼠感染甲型流感病毒肺小血管内血栓形成[J].中国比较医学杂志,2012,(2):1~5.

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