Effect of apigenin on oxidative stress in Parkinson’s disease model cells and its mechanism
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1.Department of Neurology, Weifang People’s Hospital, Weifang 261000, China. 2. Department of Gynecology, Weifang Hospital of Traditional Chinese Medicine, Weifang 261000. 3. Department of Geriatrics, Weifang Hospital of Traditional Chinese Medicine, Weifang 261000. 4. Department of Orthopedics, Weifang Hospital of Traditional Chinese Medicine, Weifang 261000. 5. Shandong Department of Neurology, First Affiliated Hospital of First Medical University, Jinan 250013

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R-33

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    Abstract:

    Objective To study the effect of apigenin ( AGN) on autophagy and oxidative stress, and its neuroprotection in MPP+ -induced Parkinson’ s model cells. Methods CCK-8 assays were used to screen the effective concentration of AGN. SH-SY5Y cells were divided into six groups: ① Control group; ② MPP+ group; ③ group A: 10 μmol / L AGN; ④ group B: 20 μmol / L AGN; ⑤ group C: 40 μmol / L AGN; ⑥ group D: 100 μmol / L AGN. After determining the effective concentration of AGN, the cells were divided into three groups: Control, MPP+ , and AGN+MPP+ groups. Apoptosis, protein expression of Caspase 3, Bcl-2, Bax, LC3-II, LC3-I, Beclin-1, and ULK-1, and propylene glycol, superoxide dismutase, and glutathione peroxidase levels were examined. Analyze each group of GFP-LC3 spots. Results AGN at 40 μmol / L significantly reduced the cytotoxic damage induced by MPP+ . Therefore, 40 μmol / L AGN was chosen as the optimal protective concentration for subsequent experiments. The apoptosis rate of the AGN+MPP+ group was lower than that of the MPP+ group ( P< 0.05). Compared with the MPP+ group, expression of Caspase 3 was decreased and the the Bcl-2/ Bax ratio was increased in the AGN+MPP+ group (all P< 0.01). Compared with the MPP+ group, expression of MDA was inhibited (P < 0. 05) and the activities of GPX and SOD were restored in the AGN+MPP+ group (P< 0.05). Intracellular GFP-LC3 spots in the AGN+MPP+ group were significantly reduced compared with those in the MPP+ group (P< 0.05). Compared with the MPP+ group, the LC3-II/ LC3-I ratio and expression of Beclin-1 and ULK1 were significantly reduced in the AGN+MPP+ group (all P< 0.05). Conclusions AGN alleviates apoptosis of SH- SY5Y cells induced by MPP+ and enhances their antioxidant capacity. Its cytoprotective effect may be related to reduction of autophagy.

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History
  • Received:May 27,2021
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  • Online: January 28,2022
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