Objective To establish a rat model of atherosclerosis with qi stagnation and blood stasis syndrome, toprovide an evaluation tool for a pharmacodynamic study of Traditional Chinese Medicine formulas. Methods According torelevant materials and references, we extracted diagnosis and treatment indicators for evaluating rat models of atherosclerosiswith qi stagnation and blood stasis syndrome. Simvastatin tablets and Tongmai granules were used as verification drugs.Based on the etiology and pathogenesis together with modern research on the pathogenesis of atherosclerosis, we used coinductionmethod in an ice-water bath, followed by fat emulsion gavage and injection of bovine serum albumin via the tailvein, to establish a rat model of atherosclerosis with qi stagnation and blood stasis syndrome. We examined the symptomsand signs and assessed the scores of rats in each group. We also measured the blood lipids and coagulation levels, detectedthe pathological changes in the ascending aorta of rats in each group after 8 weeks, and performed model establishementpre-testing. After the model was successfully established, intervention with verification drugs was performed, and the bloodlipids and the coagulation levels were measured, followed by examination of the pathological changes in ascending aorta andhepatic tissue of the rats. Results After 21 weeks, the rats’ symptoms and signs were significantly altered, the blood lipidlevels and fibrinogen levels were significantly increased, the activated partial prothrombin duration was significantlyreduced, and lipid deposition occurred in the ascending aorta intima. Furthermore, we observed vacuolation anddegeneration of hepatocytes, which was consistent with the clinical manifestations of atherosclerosis with qi stagnation andblood stasis syndrome. Compared with the model group, the blood lipids level and fibrinogen content were significantlydecreased, and no obvious abnormalities were found in the ascending aorta intima and hepatic tissue of rats in the drugverificationgroup. Conclusions The rat model of atherosclerosis with qi stagnation and blood stasis syndrome isestablished by co-induction method in an ice-water bath, fat emulsion gavage and injection of bovine serum albumin via thetail vein for continuous modeling over 21 weeks. This model has the characteristics of a high modeling rate and have clinicalsymptoms and signs. Thus, it may provide an evaluation tool for the pharmacodynamic studies of Traditional Chinese Medicine formulas against atherosclerosis with qi stagnation and blood stasis syndrome.