Objective To establish a hyperlipidemic cynomolgus monkey model with human diseasecharacteristics, and to provide an ideal animal model for the development of lipid-lowering drugs, especially lipid-loweringbiopharmaceuticals. Methods The male cynomolgus monkeys in different treatment groups were gavaged with a formulatedsemi-liquid high fat/ high sugar dietary emulsion 6 days per week. Body weight (BW), body mass index (BMI), serumtotal cholesterol ( TC), triglyceride ( TG), high density lipoprotein cholesterol ( HDL-C), low density lipoproteincholesterol (LDL-C), and other indexes were monitored regularly, and changes in the blood lipid profiles were analyzed.Results Compared with the corresponding control group, by the third month of modeling, all six middle-aged and old malecynomolgus monkeys and five adult male cynomolgus monkeys in the treatment group were successfully modeled. By the 21stmonth, all male cynomolgus monkeys in the treatment group still maintained a relatively high level of blood lipid.Conclusions A hyperlipidemic cynomolgus monkey model is successfully created through quantitative gavage using a highsugar and high fat dietary emulsion for 3 months. The model demonstrates the characteristics of hypercholesterolemia andhigh-density lipoprotein cholesterolemia, and it can be used to study the pathogenesis of hyperlipidemia and for pre-clinical drug evaluations of pharmacodynamics.