| 王孟迪,张秋梅,范 蓓,Alberto Carlos Pires Dias,王凤忠,王 琼.基于文献数据库的 6-OHDA 帕金森病大鼠模型特点分析及在中药研究中的应用[J].中国比较医学杂志,2023,33(1):93~102. |
| 基于文献数据库的 6-OHDA 帕金森病大鼠模型特点分析及在中药研究中的应用 |
| Analysis on the characteristics of 6-OHDA induced rat model of Parkinson’s disease based on literature data base and the application in the study of traditional Chinese medicine |
| 投稿时间：2021-11-15 |
| DOI：10. 3969 / j.issn.1671-7856. 2023. 01. 012 |
| 中文关键词: 帕金森病 大鼠 6-羟基多巴胺(6-hydroxydopamine, 6-OHDA) 中药 |
| 英文关键词:Parkinson’s disease rat 6-hydroxydopamine(6-OHDA) traditional Chinese medicine |
| 基金项目: |
| 作者 | 单位 | | 王孟迪 | 1.中国农业科学院农产品加工研究所,北京 100193
2.西南医科大学附属中医医院中葡中医药国际合作中心, 四川 泸州 646000
| | 张秋梅 | 1.中国农业科学院农产品加工研究所,北京 100193
2.西南医科大学附属中医医院中葡中医药国际合作中心, 四川 泸州 646000
| | 范 蓓 | 中国农业科学院农产品加工研究所,北京 100193 | | Alberto Carlos Pires Dias | 葡萄牙米尼奥大学生物系,分子和环境生物学中心,中葡药食植物资源研究中心, 葡萄牙 布拉加 4710-057 | | 王凤忠 | 中国农业科学院农产品加工研究所,北京 100193 | | 王 琼 | 1.中国农业科学院农产品加工研究所,北京 100193
2.西南医科大学附属中医医院中葡中医药国际合作中心, 四川 泸州 646000
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| Author Name | Affiliation | | WANG Mengdi | 1. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. 2. Sino-Portugal TCM International Cooperation Center, the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000. | | ZHANG Qiumei | 1. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. 2. Sino-Portugal TCM International Cooperation Center, the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000. | | FAN Bei | Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | Alberto Carlos Pires Dias | Sino-PT Research Center for Medicinal and Food Plant Resources, Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga 4710-057, Portugal | | WANG Fengzhong | Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | WANG Qiong | 1. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. 2. Sino-Portugal TCM International Cooperation Center, the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000. |
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| 中文摘要: |
| 目的 分析 6-OHDA 诱导的大鼠 PD 模型的品系选择、性别、造模要素、模型评价方法以及在抗 PD 中药研究中的应用情况,以期为 6-OHDA 模型在帕金森研究中的应用提供参考。 方法 查阅近 20 年来中国知网 (CNKI)和 PubMed 中应用 6-OHDA 建立 PD 大鼠模型的相关文献,分析 6-OHDA 诱导的大鼠 PD 模型的品系选择、性别体重、造模要素、模型评价方法以及在抗 PD 中药研究中的应用情况。 结果 6-OHDA 诱导的大鼠 PD 模型多选用雄性 SD 或 Wistar 大鼠,造模时注射部位多为单侧黑质、内侧前脑束或纹状体,多在造模 4 周后检测阿扑吗啡诱导旋转实验、脑组织形态学、分子生物学、生化指标以判断模型成功率及防治 PD 药物的有效性。 结论 本文通过对 6-OHDA 诱导的大鼠 PD 模型的品系选择、性别、体重、造模要素、模型评价方法以及在抗 PD 中药研究中的应用情况进行分析,为 PD 模型的造模和应用提供参考。 |
| 英文摘要: |
| Objective To analyze the strain selection, sex, modeling key points, evaluation method of 6- hydroxydopamine(6-OHDA) induced Parkinson’ s disease ( PD) model in rats and its application on the screening of traditional Chinese medicine in the treatment of Parkinson’ s disease. Methods Recent literatures related to 6-OHDA models in the CNKI and PubMed databases in 20 years were collected. The strains of rats, the weight, sex, modeling key points, evaluation method were analyzed, along with the application of 6-OHDA model in anti-PD traditional Chinese medicine screening. Results Male SD or Wistar rats are mostly selected as PD models in rats induced by 6-OHDA. The injection site is usually unilateral substantia nigra, medial forebrain bundle or striatum. Apomorphine induced rotation experiment, brain histomorphology, molecular biology and biochemical indicators are usually detected 4 weeks after modeling to determine the success rate of the model and the effectiveness of drugs for prevention and treatment of PD. Conclusions This study provides a useful reference for the use of 6-OHDA induced PD model regarding the strain selection, sex, modeling key points, evaluation method and the application on the screening of traditional Chinese medicine in the treatment of PD. |
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