| 许佳欢,别亚男,陈千晴,陈柏羽,欧宝芳,谢水林,吴少瑜.两种造模方法建立小鼠先兆子痫模型比较研究[J].中国比较医学杂志,2023,33(1):34~41. |
| 两种造模方法建立小鼠先兆子痫模型比较研究 |
| A comparative study of the establishment of mouse pre-eclampsia models |
| 投稿时间：2022-03-01 |
| DOI：10. 3969 / j.issn.1671-7856. 2023. 01. 005 |
| 中文关键词: 先兆子痫 脂多糖 雷西莫特 动物模型 |
| 英文关键词:preeclampsia lipopolysaccharide Resiquimod animal model |
| 基金项目: |
|
| Author Name | Affiliation | | XU Jiahuan | School of Pharmacy, Southern Medical University, Guangzhou 510515, China | | BIE Yanan | School of Life Science and Biopharmaceuticals, Guangdong Pharmaceutical University, Guangzhou 511436 | | CHEN Qianqing | School of Pharmacy, Southern Medical University, Guangzhou 510515, China | | CHEN Baiyu | School of Pharmacy, Southern Medical University, Guangzhou 510515, China | | OU Baofang | School of Pharmacy, Southern Medical University, Guangzhou 510515, China | | XIE Shuilin | School of Bioscience and Engineering, South China University of Technology, Guangzhou 510006 | | WU Shaoyu | School of Pharmacy, Southern Medical University, Guangzhou 510515, China |
|
| 摘要点击次数: 426 |
| 全文下载次数: 127 |
| 中文摘要: |
| 目的 对比分析小鼠先兆子痫(pre-eclampsia,PE)模型两种造模方法的差异,为不同类型先兆子痫动物模型的选择提供参考。 方法 将 24 只 CD-1 孕鼠随机分成 4 组:脂多糖对照组、脂多糖组、雷西莫特(R848)对照组、R848 组,每组 6 只。 脂多糖对照组和脂多糖组于妊娠第 13、14、15、16、17 天分别尾静脉注射生理盐水或 LPS (4 mg / kg);R848 对照组和 R848 组于妊娠第 13、15、17 天分别腹腔注射 R848 溶剂(5% DMSO+40% PEG300+5% Tween-80+50% saline)或 R848(10 mg / kg);同时于妊娠第 12、14、16、18 天测量尾动脉收缩压;给药后于妊娠第 18 天解剖,进行尿蛋白/尿肌酐检测、抗血管生成因子检测及 HE 染色。 结果 与对照组相比,两种造模方法均出现妊 娠期高血压(LPS 组的妊娠第 14、16、18 天的血压分别为:(142. 16±4. 81)mmHg、(144. 07±2. 91)mmHg 和(143. 31± 4. 61)mmHg;R848 组的妊娠第 14、16、18 天的血压分别为:(154. 00±5. 29)mmHg、(147. 44±3. 24)mmHg 和(140. 77 ±2. 00)mmHg);尿蛋白/ 尿肌酐检测结果显示,LPS 模型组比值升高(P<0. 05),R848 组无统计学差异(P>0. 05); 血清抗血管生成因子检测结果显示,与对照组相比,脂多糖组 sFlt-1 和 sEng 表达量无统计学差异(P>0. 05),R848 组血清内 sFlt-1 和 sEng 表达量均升高(P<0. 01);HE结果显示,与对照组相比,R848组出现孕鼠胎盘血管慢性损伤,合胞体滋养层广泛增生。 结论 两种造模方法均能使孕鼠出现高血压、胎儿生长受限、内皮功能障碍等 PE 症状。 腹腔注射 R848 造模法较尾静脉注射 LPS 法对动物的损伤程度更严重,且给药方式为腹腔注射,操作简单。 |
| 英文摘要: |
| Objective To compare and analyze differences between two modeling method of mouse pre-eclampsia (PE), and to provide a reference to select different types of PE animal models. Methods Overall, 24 CD-1 pregnant mice were randomly divided into LPS control, LPS, R848 control and R848 groups with six mice in each group. Saline or LPS (4 mg / kg) was injected intravenously via the tail vein into mice in LPS control or LPS groups on days 13 ~ 17 of pregnancy. R848 solvent or R848 (10 mg / kg) was injected intraperitoneally into mice of R848 control and R848 groups on days 13, 15 and 17 of pregnancy. Systolic blood pressure of the tail artery was measured on days 12, 14, 16 and 18 of pregnancy. The mice were dissected on day 18 of pregnancy, followed by urine protein / creatinine detection, anti-vascular generation factor detection, and HE staining. Results Compared with the control group, gestational hypertension occurred in both modeling method. Blood pressure on days 14, 16 and 18 of pregnancy in the LPS group was ( 142. 16±4. 81), (144. 07±2. 91), and (143. 31±4. 61) mmHg, respectively. Blood pressure on days 14, 16 and 18 of pregnancy in the R848 group was ( 154. 00 ± 5. 29), ( 147. 44 ± 3. 24) and ( 140. 77 ± 2. 00) mmHg, respectively. Urine protein / urine creatinine tests showed that the ratio was increased in the LPS model group ( P< 0. 05), but there was no statistical difference in the R848 group ( P> 0. 05). Compared with the corresponding control group, there was no significant difference in sFlt-1 or sEng expression in the lipopolysaccharide group (P>0. 05), whereas sFlt-1 and sEng expression was increased in the R848 group (P<0. 01). Compared with the control group, the R848 group had chronic injury of placental blood vessels, and extensive hyperplasia of syncytiotrophoblasts in pregnant mice. Conclusions Both modeling method induce symptoms of PE in pregnant mice, such as hypertension, fetal growth restriction, and endothelial dysfunction. However, the damage caused by the R848 modeling method is more serious and its advantage is a simple operation. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
