Objective To establish a stable mouse burn wound sepsis model, determine its pathophysiological indicators, and provide a standardized animal model for the study of burn sepsis. Methods The back of a mouse was exposed for 8 s to water with a temperature of 90℃ to establish a third degree scald model, and the scald area was about 15% ~ 20%. Two hours later, 100 μL of Pseudomonas aeruginosa (PA) with a concentration of 0. 75×10⁵ colony-forming units/ mL was injected under the eschar to create wound sepsis. The bodyweight, feed intake, survival rate and general status of the mice were observed and recorded for 12 days. Peripheral blood and liver tissues were collected from the mice on post-infection days 1, 2, 3, 5 and 7, the serum biochemical and inflammatory factors were measured, and the pathological changes of liver tissues were observed. Results The mortality of the model mice on the twelfth day post- infection was 30. 2%. The levels of the serum biochemical indexes ALT, AST, UREA and CREA were significantly increased from baseline (P<0.05 or P<0.01), whereas the levels of TP and TBIL exhibited no obvious changes. The serum inflammatory indexes TNF-α and IL-6 increased to their highest values on the first day after infection (P<0.01) and then decreased daily, recovering to their baseline levels on the third and seventh days post-infection, respectively. The histopathological sections of liver revealed liver injury at all tested timepoints. Conclusions A simple burn sepsis model with a manageable protocol and appropriate severity was established; the model characteristics are in line with the pathophysiological characteristics of burn sepsis; and this approach can provide a standardized experimental animal model both for relevant mechanism research and for drug research and development.