芹菜素对帕金森模型细胞氧化应激水平的影响及机制的研究
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1.潍坊市人民医院神经内科,山东 潍坊 261000;2.潍坊市中医院妇科,山东 潍坊 261000; 3.潍坊市中医院老年科,山东 潍坊 261000;4.潍坊市中医院骨科,山东 潍坊 261000; 5.山东第一医科大学第一附属医院神经内科,济南 250013

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R-33

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Effect of apigenin on oxidative stress in Parkinson’s disease model cells and its mechanism
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1.Department of Neurology, Weifang People’s Hospital, Weifang 261000, China. 2. Department of Gynecology, Weifang Hospital of Traditional Chinese Medicine, Weifang 261000. 3. Department of Geriatrics, Weifang Hospital of Traditional Chinese Medicine, Weifang 261000. 4. Department of Orthopedics, Weifang Hospital of Traditional Chinese Medicine, Weifang 261000. 5. Shandong Department of Neurology, First Affiliated Hospital of First Medical University, Jinan 250013

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    摘要:

    目的 研究芹菜素(AGN)对 MPP+诱发的帕金森细胞模型中自噬、氧化应激的影响及神经保护作用。 方法 CCK-8 筛选 AGN 有效浓度,SH-SY5Y 细胞分 6 组:①对照组;②MPP+组;③A 组:AGN 浓度 10 μmol / L; ④B 组:AGN 浓度 20 μmol / L;⑤C 组:AGN 浓度 40 μmol / L;⑥D 组:AGN 浓度 100 μmol / L。 确定 AGN 有效浓度后 将细胞分为 3 组:对照组、MPP+组及 AGN+MPP+组。 检测细胞凋亡及 Caspase3、Bcl-2、Bax、LC3-II、LC3-I、Beclin-1、 ULK1 蛋白表达,检测丙二醇、超氧化物歧化酶和谷胱甘肽过氧化物酶水平。分析每组 GFP-LC3 斑点。 结果 AGN 在 40 μmol / L 时能显著降低 MPP+诱导的细胞毒性损伤,选择 40 μmol / L 作为最佳药物保护浓度进行后续研究。 AGN+MPP+组细胞凋亡率较 MPP+组下降(P<0. 05)。 相较于 MPP+组,AGN+MPP+组 Caspase3 表达降低,Bcl-2/ Bax 值升高(P 值均<0. 01)。 与 MPP+组比较,AGN+MPP+组抑制 MDA 的表达(P<0. 05),恢复 GPX 和 SOD 的活性(P< 0. 05)。 AGN+MPP+组细胞内 GFP-LC3 斑点较 MPP+组显著减少(P<0. 05)。对比 MPP+组,AGN+MPP+组 LC3-II/ LC3-I 比值及 Beclin-1、ULK1 表达显著降低(P 值均<0. 05)。 结论 AGN 能减轻 MPP+诱导的 SH -SY5Y 细胞凋亡,增强抗氧化能力,其细胞保护作用可能与降低细胞自噬水平相关。

    Abstract:

    Objective To study the effect of apigenin ( AGN) on autophagy and oxidative stress, and its neuroprotection in MPP+ -induced Parkinson’ s model cells. Methods CCK-8 assays were used to screen the effective concentration of AGN. SH-SY5Y cells were divided into six groups: ① Control group; ② MPP+ group; ③ group A: 10 μmol / L AGN; ④ group B: 20 μmol / L AGN; ⑤ group C: 40 μmol / L AGN; ⑥ group D: 100 μmol / L AGN. After determining the effective concentration of AGN, the cells were divided into three groups: Control, MPP+ , and AGN+MPP+ groups. Apoptosis, protein expression of Caspase 3, Bcl-2, Bax, LC3-II, LC3-I, Beclin-1, and ULK-1, and propylene glycol, superoxide dismutase, and glutathione peroxidase levels were examined. Analyze each group of GFP-LC3 spots. Results AGN at 40 μmol / L significantly reduced the cytotoxic damage induced by MPP+ . Therefore, 40 μmol / L AGN was chosen as the optimal protective concentration for subsequent experiments. The apoptosis rate of the AGN+MPP+ group was lower than that of the MPP+ group ( P< 0.05). Compared with the MPP+ group, expression of Caspase 3 was decreased and the the Bcl-2/ Bax ratio was increased in the AGN+MPP+ group (all P< 0.01). Compared with the MPP+ group, expression of MDA was inhibited (P < 0. 05) and the activities of GPX and SOD were restored in the AGN+MPP+ group (P< 0.05). Intracellular GFP-LC3 spots in the AGN+MPP+ group were significantly reduced compared with those in the MPP+ group (P< 0.05). Compared with the MPP+ group, the LC3-II/ LC3-I ratio and expression of Beclin-1 and ULK1 were significantly reduced in the AGN+MPP+ group (all P< 0.05). Conclusions AGN alleviates apoptosis of SH- SY5Y cells induced by MPP+ and enhances their antioxidant capacity. Its cytoprotective effect may be related to reduction of autophagy.

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张跃其,胡炳蕾,王默然,马海强,张 君,陈学丛.芹菜素对帕金森模型细胞氧化应激水平的影响及机制的研究[J].中国比较医学杂志,2021,31(12):77~83.

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  • 收稿日期:2021-05-27
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  • 在线发布日期: 2022-01-28
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