Objective To use the SYRCLE animal experiment risk assessment tool, ARRIVE 2019 guidelines and GSPC checklist to evaluate the report quality of animal experiments of electroacupuncture in the neurogenic bladder after spinal cord injury and determine method to improve the quality of animal experiment reports. Methods CNKI, WANFANG, VIP, SinoMed, PubMed, Web of science and Cochrane library databases were searched to find animal experiments of electroacupuncture in the neurogenic bladder in accordance with the SYRCLE animal experiment risk assessment tool, ARRIVE 2019 guidelines and GSPC list. These data were extracted from each database and statistical analysis was performed using Excel 2019. Stata software was used for systematic evaluation and analysis. Results Twenty- six studies were finally included. The total scores of all studies in the SYRCLE animal experiment risk assessment tool were generally low, which ranged from 10 to 14 points and the risk of bias was high. Only 18 studies used the “random number table method ”. No studies used blinded method in animal allocation or result measurement. There were 21 studies in which the number of animals was missing during the experiment, but none of them took measures or described the effect on experimental result. The low-risk rate of essential items in the ARRIVE 2019 guideline is 43. 14%, while the low-risk rate of recommended items is only 23. 56% and the low-risk rate of the GSPC list is 20. 45%. There were 21 studies that reported animal exclusion mostly due to death, other complications, or model failure. Eighteen studies used the random number table method and eight studies only mentioned randomness without specifying the method. Eight studies described the animal species, age, sex and weight in detail. Two studies both provided animal license numbers and indicated that they had passed ethical review. Three studies described measures to reduce pain and suffering during the experiments. Six studies discussed the limitations of the research. Nine studies described the current research progress of NGB after SCI and provided relevant references, indicated the deficiencies in current treatments and established clear research goals. Two studies blindly described specific outcome indicators and measurement method to experimental staff. The 26 studies discussed the main findings and explained the clinical significance of the result and the significance of the entire science. Systematic evaluation of the study result showed that electroacupuncture improved the maximum bladder volume and bladder compliance and reduced the bladder leakage point pressure compared with the model group. Conclusions The current publicly published animal studies on electroacupuncture of the neurogenic bladder after spinal cord injury are generally of low quality and descriptions of multiple items are incomplete, which prevents readers from Objectively and accurately assessing the level of risk of bias that the animal experiment may produce and even affects the reader’ s understanding of animal research. Judgments can be further translated into clinical research. It is recommended to take targeted measures to further promote and use SYRCLE animal experiment bias risk assessment tools and experimental research report specifications (ARRIVE 2019 guidelines and GSPC list),which can further improve the design, implementation, and reporting of animal experiments and improve the reproducibility of animal experimental research and result.