脑栓通胶囊对 ApoE-/- 小鼠主动脉炎症因子表达的影响
作者:
作者单位:

1.遵义医科大学基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室,贵州 遵义 563006; 2.首都医科大学宣武医院实验动物室,北京市老年病医疗研究中心,北京 100053

作者简介:

通讯作者:

中图分类号:

R-33

基金项目:


Effect of Naoshuantong capsule on the expression of aortic inflammatory factors in ApoE- / -mice
Author:
Affiliation:

1.Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education,Zunyi Medical University,Zunyi, Guizhou 563006, China. 2. Experimental Animal Center, Xuanwu Hospital of Capital Medical University, Beijing Municipal Geriatrics Medical Reseach Center, Beijing 100053

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 观察脑栓通胶囊对 ApoE-/- 动脉粥样硬化小鼠模型炎症相关因子白介素 1β( iterleukin-1 beta, IL-1β)、白细胞介素-6(interleukin- 6, IL- 6)、肿瘤坏死因子(tumor necrosis factor-α, TNF-α)、细胞间粘附分子-1(intercellular cell adhesion molecule-1, ICAM-1)和金属基质蛋白酶-9(matrix metalloproteinase, MMP-9)的表达, 初步探讨其治疗动脉粥样硬化的机制。 方法 8 周龄雄性 ApoE-/-小鼠,采用高脂饮食法建立动脉粥样硬化模型, 造模成功后随机分为模型对照组、脑栓通胶囊低剂量组、脑栓通胶囊高剂量组。 低剂量组给予脑栓通胶囊 0. 5 g / kg 体重,高剂量组给予脑栓通胶囊 1. 0 g / kg 体重,模型对照组给予等量的蒸馏水。 连续给药 12 周后,红油 O 染色检测整体主动脉内膜斑块,观察比较各组小鼠的炎症相关因子 IL-1β、IL-6、TNF-α、ICAM-1 和 MMP-9 的表达。 结果 药物给予干预后,脑栓通胶囊高剂量组的主动脉斑块面积显著低于模型对照组(P<0. 01)。 与模型对照组相比, 脑栓通胶囊低剂量组能够分别抑制炎症相关因子 IL-1β 和 IL-6 的蛋白表达水平(P<0. 05),脑栓通高剂量组中的 IL-1β、IL-6、TNF-α 和 ICAM-1 表达均有所降低(P<0. 05,P<0. 01)。 MMP-9 的表达与模型对照组比较有降低的趋势,但无统计学意义(P>0. 05)。 结论 脑栓通胶囊可以调节炎症相关因子的表达水平,其治疗动脉粥样硬化的作用机制很可能与该途径有关。

    Abstract:

    Objective To investigate the effects of Naoshuantong capsule on the expression of inflammatory cytokines IL-1β, IL-6, ICAM-1, MMP-9 and TNF-α in the atherosclerosis ApoE-/-mouse model and to explore therapeutic mechanisms. Methods Eight-week-old ApoE-/- male mice were fed a high-fat diet to establish atherosclerosis. After the diet, the mice were randomly divided into the atherosclerosis control group, Naoshuantong capsule low-dose group and Naoshuantong capsule high-dose group. The low-dose and high-dose groups were given 0. 5 and 1. 0 g / kg Naoshuantong capsule, respectively, and the control group was given an equivalent volume of distilled water. After 12 weeks, whole-aortic intimal plaques were detected by oil red O staining, and protein levels of inflammation-related factors IL-1β, IL-6, ICAM- 1, MMP-9 and TNF-α were observed and compared between each group. Results After drug intervention, the aortic plaque area of the high-dose group was significantly lower than that of the control group (P< 0. 01). Compared with the atherosclerosis control group, protein expression levels of IL-1β and IL-6 were decreased in the low-dose group ( P< 0. 05), and IL-1β, IL-6, ICAM-1 and TNF-α levels were markedly reduced in the high-dose group (P<0. 05,P<0. 01). The expression of MMP-9 was not significantly different compared with the control group ( P> 0.05). Conclusions Naoshuantong capsule regulated the expression of inflammatory-related factors, and its potential mechanism for the treatment of atherosclerosis may be related to inflammatory-related pathways.

    参考文献
    相似文献
    引证文献
引用本文

李子洁,刘婷婷,赵学庆,郑文荣,王宇峰,田 欣,祝自新,孙芳玲,王 文.脑栓通胶囊对 ApoE-/- 小鼠主动脉炎症因子表达的影响[J].中国比较医学杂志,2021,31(5):36~40.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2020-09-01
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2021-06-25
  • 出版日期:
自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
关闭