Abstract: Objective To investigate the effects of Naoshuantong capsule on the expression of inflammatory cytokines IL-1β, IL-6, ICAM-1, MMP-9 and TNF-α in the atherosclerosis ApoE-/-mouse model and to explore therapeutic mechanisms. Methods Eight-week-old ApoE-/- male mice were fed a high-fat diet to establish atherosclerosis. After the diet, the mice were randomly divided into the atherosclerosis control group, Naoshuantong capsule low-dose group and Naoshuantong capsule high-dose group. The low-dose and high-dose groups were given 0. 5 and 1. 0 g / kg Naoshuantong capsule, respectively, and the control group was given an equivalent volume of distilled water. After 12 weeks, whole-aortic intimal plaques were detected by oil red O staining, and protein levels of inflammation-related factors IL-1β, IL-6, ICAM- 1, MMP-9 and TNF-α were observed and compared between each group. Results After drug intervention, the aortic plaque area of the high-dose group was significantly lower than that of the control group (P< 0. 01). Compared with the atherosclerosis control group, protein expression levels of IL-1β and IL-6 were decreased in the low-dose group ( P< 0. 05), and IL-1β, IL-6, ICAM-1 and TNF-α levels were markedly reduced in the high-dose group (P<0. 05,P<0. 01). The expression of MMP-9 was not significantly different compared with the control group ( P> 0.05). Conclusions Naoshuantong capsule regulated the expression of inflammatory-related factors, and its potential mechanism for the treatment of atherosclerosis may be related to inflammatory-related pathways.