Objective To observe the dose-effect relationship of xingnaojing injection in treating coma in beagle dogs. Methods Male beagles were divided into nine groups at random: Control, Model, Naloxone ( 0. 01 mg / kg),Xingnaojing group (dose 1) (0. 215 mL/ kg), Xingnaojing group (dose 2) (0. 43 mL/ kg), Xingnaojing group ( dose 3, single dose) ( 0. 86 mL/ kg), Xingnaojing group ( dose 3, double dose) (( 0. 43 + 0. 43) mL/ kg), Xingnaojing group (dose 4, single dose) ( 1. 29 mL/ kg), and Xingnaojing group ( dose 4, double dose) (( 0. 65 + 0. 65) mL/ kg). Lu mianning was used for anesthesia, fixed in a custom-made hammer, and a model of traumatic brain injury in dogs was established. MRI was performed, intravenous medication for 14 days. The waking time, coma degree, and cortical electroencephalogram were measured. After the last intravenous administration of medication, MRI was performed and blood was taken to determine liver and kidney function, along with routine blood tests and measurements of NSE and S-100B. Cerebrospinal fluid was used to measure Glu, GABA, and His. Histopathological examination was performed on the heart, liver, spleen, lung, kidney, adrenal gland, brain, pituitary, sciatic nerve, and spinal nerve from animals in each group, and some organs were weighed. Results Xingnaojing injection doses 1, 2 (single dose), and 3 (two doses) significantly reduced the waking time of dogs. Xingnaojing doses 1, 2, and 4 significantly reduced the levels of S100B and NSE in serum. Xingnaojing doses 1, 2, 3 (2 doses), 4, and 5 (2 doses) significantly increased the area of brain injury recovery in dogs. The numbers of lymphocytes and eosinophils in xingnaojing injection group 1 and group 2 significantly decreased. Xingnaojing injection dose 1 significantly reduced the number of mononuclear cells in dogs. Xingnaojing injection doses 4 and 5 had no significant effect on canine blood routine findings. There were no significant differences in ALT, AST, TBil, BUN, and CRE between the xingnaojing injection groups. Xingnaojing injection showed no significant difference in histopathology of canine heart, liver, spleen, lung, kidney, and brain. Conclusions Xingnaojing injection clearly ameliorated the coma caused by traumatic brain injury in beagle dogs, while having no clear toxic effects at a high dose.