Objective This study was performed to establish a method of measurement of blood pressure, electrocardiogram (ECG) parameters, and respiration in conscious Beagle dogs by an implantable physiological telemetry technique. Methods (1) The model dogs were established by surgical implantation of a vascular access port into one side of the carotid artery. After animal recovery, the blood pressure, ECG, and respiratory indexes of six model dogs were tested once a week for 4 weeks to verify the stability of the physiological telemetry system. (2) The differences in the blood pressure, ECG, and respiratory indexes between model dogs and anesthetized dogs were compared. (3) Model dogs were given positive drugs to verify the sensitivity of the physiological telemetry system. Results (1) No significant differences were found in blood pressure, ECG, or and respiratory indexes among the four measurement time points in model dogs, and the indexes were stable. (2) The blood pressure and heart rate were significantly higher, the PR interval was shorter, the QTc interval was significantly longer, and the respiratory frequency and respiratory amplitude were lower in the anesthetized dogs than in the model dogs. (3) The diastolic, systolic, and mean blood pressure of the model dogs were significantly lower after azilsartan administration. After terfenadine administration, the dogs’ heart rate decreased and the PR interval, QRS, and QTc interval were prolonged. Conclusions The herein-described physiological telemetry system is successfully established in Beagle dogs and shows good repeatability, high sensitivity, and reliable data. This system may be useful for pharmacological drug safety research.