Objective To investigate appropriate nutritional intervention measures to reduce the adverse effects onexperimental tree shrews caused by stress responses after long-distance transport. Methods First, based on their diet inthe wild, we fed healthy adult tree shrews fresh fruits and vegetables in our center, recorded their weight change, activestate, and the amounts of residual food, to find the optimal combination of nutritional options that meet their energyexpenditure demands and food preference. Next, tree shrews which were after long-distance transportation stress wererandomly divided into three groups, and put on nutrition programs, containing conventional feed and adequate amounts offruit and vegetables, to evaluate whether the nutritional intervention method is effective. To this end, we analyzed theirsurvival rate, mental state and weight change. Results Tree shrews prefer fruits with higher sugar content such as applesand pears. Mealworm had a significant effect on the weight gain of tree shrews ( P = 0. 019). An adult male tree shrewconsumes about 60 g of Fuji apples + 10 g of dry breadworm + 15 g of standard feed a day. The mortality of tree shrews canbe effectively reduced by nutritional intervention ( P = 0. 02). Conclusions The nutritional intervention program reducesthe mortality of tree shrews during stress responses after long-distance transportation, and helps the recovery of their mental state.