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万雪超,卢秋芬,何玉玲,方艳.妊娠糖尿病母鼠胎儿心脏组织CD4+ T 细胞水平表达及相关机制研究[J].中国比较医学杂志,2019,29(8):93~98.
妊娠糖尿病母鼠胎儿心脏组织CD4+ T 细胞水平表达及相关机制研究
Down-regulation of CD4+ T cells in fetal heart tissue of rats with gestational diabetes mellitus
投稿时间:2018-11-22  
DOI:10.3969/j. issn. 1671 -7856. 2019. 08. 015
中文关键词:  糖尿病  妊娠期  CD4+ T 细胞  心脏组织  miR-233-3p
英文关键词:diabetes mellitus  pregnancy  CD4+ T cells  cardiac tissue  miR-233-3p  rat
基金项目:
作者单位E-mail
万雪超 贵州省铜仁市人民医院,贵州铜仁 554300 wql6662a@ 126.com 
卢秋芬 贵州省铜仁市人民医院,贵州铜仁 554300  
何玉玲 贵州省铜仁市人民医院,贵州铜仁 554300  
方艳 贵州省铜仁市人民医院,贵州铜仁 554300  
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中文摘要:
      目的 探讨分析妊娠糖尿病母鼠胎儿心脏组织CD4+ T 细胞水平表达及其相关机制?方法 将怀孕的84 只雌性SD 大鼠随机分为正常对照组(NC 组)和STZ 诱导的妊娠糖尿病模型组(gestational diabetes mellitus,GDM 组),免疫组化检测不同组别胎鼠心脏组织中CD4+ T 细胞水平, Western blotting 检测IL-17?IL-4?Tim-3?PD-1表达,RT-PCR 检测miR-223-3p 表达?结果 GDM 组CD4+ CD25+ Treg 阳性细胞表达数量[(46. 41±10. 94)%]低于NC 组[(21. 63±10. 94)%],差异有统计学意义( P <0. 01)?GDM 组IL-17?Tim-3 及PD-1 表达量均高于NC 组,而IL-4 低于NC 组,差异有统计学意义( P <0. 05 或 P <0. 01)?GDM 组miR-233-3p 相对表达量(0. 682±0. 104)低于NC 组(0. 466±0. 117),差异有统计学意义( P <0. 05)?结论 GDM 子鼠心脏组织异常可能与CD4+ T 细胞表达下降有关,而这一作用机制可能与胎鼠心脏组织中miR-233-3p 表达下调及其介导的炎症反应有关?
英文摘要:
      Objective To investigate the level of CD4+ T cells and related mechanisms in fetal heart tissue fromfemale rats with gestational diabetes mellitus ( GDM). Methods Eighty pregnant female Sprague-Dawley rats wererandomly divided into normal control (NC) group and GDM group induced by streptozotocin. CD4+ cell levels were detectedin the fetal heart tissues of different groups. Western blotting was performed to detect the interleukin (IL)-17, IL-4, T-cellimmunoglobulin mucin (Tim)-3, and programmed cell death protein (PD)-1 expressions, and real-time PCR was used todetect the expression of miR-233-3p. Results The expression of CD4+ CD25+ Treg cells in the GDM group (46. 41±10. 94%) was significantly higher than in the NC group (21. 63±10. 94%) ( P <0. 01). Expression levels of IL-17, Tim-3, and PD-1 were also significantly higher in the GDM group than in the NC group, while the expression of IL-4 wassignificantly lower ( P <0. 05 or P <0. 01). The relative expression of miR-233-3p in the GDM group (0. 682±0. 104) wassignificantly lower than in the NC group (0. 466±0. 117) ( P <0. 05). Conclusions Cardiac tissue abnormalities in GDMrats may be associated with the down-regulated expression of CD4+ T cells, and the underlying mechanism may be associated with the down-regulation of miR-233-3p expression in the fetal rat heart tissue.
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