急性运动对大鼠心肌miRNA-30a 表达的影响
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(1.首都体育学院研究生部,北京 100191; 2.首都体育学院运动科学与健康学院,北京 100191)

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R-33

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Expression of miRNA-30a in rat myocardium induced by acute exercise
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(1. Department of Postgraduates, Capital University of Physical Education and Sports, Beijing 100191, China.2. School of Sport Science and Health, Capital University of Physical Education and Sports, Beijing 100191)

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    摘要:

    目的 研究急性运动对大鼠心肌miRNA-30a 及其对自噬相关基因表达的影响,探讨miRNA-30a 对运动后不同恢复时期心肌自噬活性的调控机制?方法 SD 大鼠随机分为安静对照组(Con),运动后0 h 组(E0),运动后3 h 组(E3)和运动后12 h 组(E12)?急性运动后按不同时间点取材?使用实时荧光定量聚合酶链反应(PCR)检测左心室肌中ACE mRNA?miRNA-30a?Beclin-1 mRNA 和LC3 mRNA 表达量?结果 与安静对照组相比,运动后0 h 组左心室肌中ACE mRNA 和miRNA-30a 表达显著性升高( P <0. 05),Beclin-1 mRNA 和LC3 mRNA 表达也显著提高( P <0. 05),在运动后3 h 均恢复到安静水平?结论 急性运动可以显著提高心肌ACE mRNA?miRNA-30a?Beclin-1 mRNA 和LC3 mRNA 表达,这种一过性升高可能是由运动应激导致的?

    Abstract:

    Objective To study the influence of acute exercise on rat myocardial microRNA-30a and autophagyrelatedgene expression, and investigate the possible mechanism through which microRNA-30a regulates myocardial autophagyduring different recovery periods after exercise. Methods SD rats were randomly divided into a control group (Con), a 0 hafter exercise group (E0), a 3 h after exercise group (E3), and a 12 h after exercise group (E12). Real-time fluorescencequantitative polymerase chain reaction was used to detect the expression of ACE mRNA, microRNA-30a, Beclin-1 mRNA,and LC3 mRNA in the left ventricular myocardium. Results Compared with the control group, in the E0 group, ACE mRNAand microRNA-30a gene expression in the left ventricular muscle was significantly increased ( P <0. 05), and Beclin-1 mRNAand LC3 mRNA expression also significantly increased ( P <0. 05). This was recovered to resting state approximately 3 h afterexercise. Conclusions Acute exercise can significantly improve myocardial ACE mRNA, microRNA-30a, Beclin-1 mRNA and LC3 mRNA expression. This transient increase may be caused by exercise-induced stress.

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杨赟,王智强,王蕴红.急性运动对大鼠心肌miRNA-30a 表达的影响[J].中国比较医学杂志,2019,29(7):76~79.

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  • 收稿日期:2018-11-09
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  • 在线发布日期: 2019-08-01
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