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吴璟,郑婕,金学琴,黎伟华.葫芦巴碱对热应激致小鼠睾丸损伤的保护作用[J].中国比较医学杂志,2019,29(7):47~52.
葫芦巴碱对热应激致小鼠睾丸损伤的保护作用
Protective effect of trigonelline on testicular tissue injury induced by heat stress in mice
投稿时间:2018-12-27  
DOI:10.3969/j. issn. 1671 -7856. 2019. 07. 008
中文关键词:  葫芦巴碱  热应激  脏器系数  睾丸组织  小鼠
英文关键词:trigonelline  heat stress  organ coefficient  testicular tissue  mouse
基金项目:
作者单位E-mail
吴璟 宁夏医科大学实验动物中心,银川 750004 wj.nxmc@ qq.com 
郑婕 宁夏医科大学药学院,银川 750004  
金学琴 宁夏医科大学实验动物中心,银川 750004  
黎伟华 宁夏医科大学实验动物中心,银川 750004 546240233@ qq.com 
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中文摘要:
      目的 研究葫芦巴碱对热应激致小鼠睾丸损伤的保护作用?方法 雄性ICR 小鼠120 只,随机分为正常对照组?模型组?葫芦巴碱药物干预组(25 mg/ kg,50 mg/ kg,100 mg/ kg),每组24 只?各组小鼠给予相应药物灌胃一周后,除正常对照组外,将其余各组小鼠下腹部置于43℃恒温水浴锅中热应激处理15 min,随后药物干预组给予相应剂量葫芦巴碱灌胃给药14 d,正常对照组和模型组给予生理盐水灌胃14 d,并分别于热刺激处理后1,7,10,14 d 取材?计算生殖器官指数,精子数,HE 染色观察睾丸组织结构变化,并进行比较分析?结果 与正常对照组相比,模型组小鼠生殖器官脏器系数?附睾精子数均显著降低( P <0. 05),病理组织学显示小鼠睾丸损伤明显?与模型组比较,各剂量药物干预组均能升高小鼠生殖器官脏器系数及附睾精子数量( P <0. 05);病理组织学显示各剂量药物干预组均能明显改善热应激诱导的小鼠睾丸损伤?结论 葫芦巴碱能够明显改善热应激对小鼠睾丸的损伤程度,具有一定的保护作用?
英文摘要:
      Objective To study the protective effect of trigonelline on testicular injury induced by heat stress inmice. Methods A total of 120 ICR male mice were randomly divided into a control group, model group, and trigonellinegroups (25 mg/ kg, 50 mg/ kg, 100 mg/ kg), with 24 rats in each group. The lower abdomen of the mice in the model andtrigonelline groups was placed in a constant temperature water bath at 43℃ for 15 min to induce heat stress, and then thetrigonelline group was treated using trigonelline through intragastric gavage for 14 days. The mice in the control group andthe model group were treated using saline for 14 days. Animals were sacrificed at 1, 7, 10, and 14 days after heatstimulation. The genital organ index, a sperm count, and HE staining were used to observe changes in testicular tissuestructure and for a comparative analysis. Results Compared with the control group, the genital organ coefficient and thenumber of epididymal sperm in the model group were significantly decreased ( P < 0. 05). A histopathological analysisshowed obvious testicular injury in the mice. Trigonelline treatment increased the genital organ coefficient and the number ofspermatozoa in the epididymis, compared with the model group ( P < 0. 05). Histopathology showed that trigonellinetreatment significantly reduced heat stress-induced injury in the mice. Conclusions Trigonelline can significantly improvethe testicular coefficient and reduce heat stress-induced damage in testis tissue in mice, and it has a certain antagonistic effect.
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