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李晓娟,李兴杰,侯俊,孙慧伟,冯帆,王志杰,李润,李瑞生.BALB/ c 突变卷毛小鼠皮肤创伤愈合对比分析[J].中国比较医学杂志,2019,29(7):42~46.
BALB/ c 突变卷毛小鼠皮肤创伤愈合对比分析
Impaired skin wound healing in BALB / c mutant curly mouse
投稿时间:2019-03-21  
DOI:10.3969/j. issn. 1671 -7856. 2019. 07. 007
中文关键词:  BALB/ c 小鼠  突变卷毛  皮肤愈合
英文关键词:BALB/ c mouse  mutant curly  skin healing
基金项目:
作者单位E-mail
李晓娟 1.中国人民解放军总医院第五医学中心临床研究管理中心,北京 100039
2.解放军医学院,北京 100853 
sxlily55@ 163.com 
李兴杰 中国人民解放军总医院第五医学中心临床研究管理中心,北京 100039 710988681@ qq.com 
侯俊 中国人民解放军总医院第五医学中心临床研究管理中心,北京 100039  
孙慧伟 中国人民解放军总医院第五医学中心临床研究管理中心,北京 100039  
冯帆 中国人民解放军总医院第五医学中心临床检验诊疗中心,北京 100039  
王志杰 中国人民解放军总医院第五医学中心临床研究管理中心,北京 100039  
李润 中国人民解放军总医院第五医学中心临床研究管理中心,北京 100039  
李瑞生 中国人民解放军总医院第五医学中心临床研究管理中心,北京 100039 lrsheng@ sohu.com 
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中文摘要:
      目的 探讨BALB/ c 突变卷毛小鼠在皮肤损伤自然愈合过程中与对照BALB/ c 小鼠存在的差异?方法 选择6 周龄BALB/ c 突变卷毛小鼠和对照BALB/ c 小鼠各10 只,于小鼠背部做2 个直径为0. 5 cm 的圆形皮肤全层创口,备皮肤损伤模型,于损伤后第3,7,10,14 天观察皮肤外形愈合情况,测量皮肤损伤面积的变化及计算伤口愈合率;同时于损伤后第3,7,10,14 天取损伤部位皮肤连带周围正常皮肤,进行HE 染色和天狼猩红染色,以观察皮肤在愈合过程中的形态学变化?结果 外观结果显示:突变卷毛小鼠在创后3 d 和7d 的愈合伤口明显愈合较快?愈合率结果显示:卷毛小鼠的皮肤在创后3 d?7d 的愈合率显著高于对照小鼠的愈合率,具有统计学意义( P <0. 05),而在创后10 d 和14 d 两组小鼠的愈合率不具有显著差异?病理结果显示:突变卷毛小鼠在创后3 d 创面胶原纤维明显增多,可见少量炎症细胞浸润,毛细血管扩张充血,创后7 d 胶原增长迅速,肉芽组织显著增多,可见部分表皮再生生长?天狼猩红染色结果显示:卷毛小鼠在创后3 d 至14 d 可以清晰的看到胶原纤维和肉芽组织从底层逐渐顶替修复破损部位,直至表皮和毛囊生长出来?结论 BALB/ c 突变卷毛小鼠皮肤创伤后前期的愈合能力强于对照小鼠,为今后该突变小鼠应用于皮肤创伤愈合模型研究提供了理论参考?
英文摘要:
      Objective To investigate the difference between BALB/ c mutant curly mice and control BALB/ cmice in terms of natural healing of skin injuries. Methods Ten 6-week-old BALB/ c mutant curly mice and 10 controlBALB/ c mice were selected. Two full-thickness skin wounds with a diameter of 0. 5 cm were created on the back of themice. A skin injury model was used. Skin healing was observed at 3, 7, 10, and 14 days after injury, and changes in theskin damage area and the wound healing rate were measured. Animals were sacrificed at 3, 7, 10, and 14 days after injury.HE staining and Sirius staining were performed to observe pathological changes in the skin during healing. Results Theskin wounds healed faster in the mutant curly mice at 3 and 7 days after wound healing. The skin wound healing rate of thecurly mice at 3 and 7 days after injury was significantly higher than that of the control mice ( P < 0. 05), but there was nosignificant difference between the two groups at 10 and 14 days after injury. The pathological examination showed that thecollagen fibers of the mutant curly haired mice increased significantly 3 days after injury, and there was a small amount ofinflammatory cell infiltration, and capillary dilation and congestion. Collagen grew rapidly 7 days after injury, levels ofgranulation tissue increased significantly, and some epidermis regenerated and grew. The Sirius scarlet staining resultshowed that collagen fibers and granulation tissue were present from the bottom layer of the injury, to repair the damagedarea, until the epidermis and hair follicles grew out from 3 to 14 days in the curly-haired mice. Conclusions BALB/ cmutant curly mice have better healing ability than control mice in the early stage of skin trauma, which provides a theoretical reference for the application of BALB/ c mutant mice in skin wound healing models in the future.
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