大鼠急性心肌缺血损伤后心脏结构和功能改变的超声心动图评价
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(中国中医科学院西苑医院基础医学研究所,北京 100091)

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R-33

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Ultrasound cardiogram assessment of cardiac structure and function in rats with acute ischemic myocardial injury
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(Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China)

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    目的 对大鼠急性心肌缺血损伤后心脏结构和功能改变进行超声心动图评价?方法 30 只雄性大鼠随机分为假手术组,模型组,地尔硫卓组,每组10 只?采用冠状动脉左前降支结扎的方法建立大鼠急性心肌缺血损伤模型?模型建立后连续给药15 d 后,采用心脏超声检测左心室结构的功能的改变,包括左心室射血分数(EF),左心室短轴缩短率(FS),每搏输出量(SV),心输出量(CO),左心室舒张末期和收缩末期内径(LVIDd 和LVIDs),舒张末期和收缩末期左心室后壁厚度(LVPWd 和LVPWs),舒张末期和收缩末期左室前壁厚度(LVAWd和LVAWs)等指标;采用伊文思蓝和TTC 染色法测定左心室扩张程度;采用ELISA 方法检测血清氨基末端脑钠肽前体(NT-proBNP)水平;并在光镜下观察各组心肌组织HE 染色的病理改变?结果 心脏超声显示,与假手术组比较,模型组大鼠LVIDd 和LVIDs 明显增加,而LVAWd?LVAWs?LVPWd 和LVPWs 明显降低(P<0. 05 或P<0. 01);同时血清NT-proBNP 水平升高,左心室腔明显扩张?与模型组比较,地尔硫卓能够明显降低大鼠心肌缺血后LVIDd 和LVIDs,增加LVAWd,LVAWs,LVPWd 和LVPWs;同时血清NT-proBNP 水平下降,抑制左心室腔扩大( P <0. 05 或 P <0. 01)?结论 心脏超声多种指标结合能够很好地评价大鼠急性心肌缺血损伤后心脏结构和功能改变,将成为小动物心血管疾病研究的主要手段?

    Abstract:

    Objective To evaluate cardiac structure and function in rats with acute ischemic myocardial injury byultrasound cardiogram. Methods SD rats were randomly divided into three groups: sham group, model group withischemic myocardial injury, and a diltiazem treatment group (10 mg/ kg). Myocardial ischemic injury was induced byligating the left anterior descending coronary artery. Left ventricular structure and function were measured by ultrasoundcardiogram after 15 d. The parameters included EF, FS, SV, CO, LVIDd, LVIDs, LVPWd, LVPWs, LVAWd andLVAWs were assessed. The serum concentration of NT-proBNP was assessed using ELISA. Left ventricular dilation wasanalyzed through Evans and TTC staining. HE staining was used for the pathological examination of myocardial structure.Results Compared with the sham group, the degree of left ventricular dilation, the serum levels of NT-proBNP, andLVIDd and LVIDs were significantly decreased in the model group, but LVPWd, LVPWs, LVAWd and LVAWs wereincreased. Diltiazem treatment decreased LVIDd and LVIDs, and increased LVAWd, LVAWs, LVPWd, and LVPWs,compared with the model group, as assessed using an ultrasound cardiogram analysis. The diltiazem group also showed asignificant decrease in serum NT-proBNP levels, and left ventricular dilation compared with the model group. ConclusionsAn ultrasound cardiogram analysis is suitable as the primary method for evaluating cardiac structure and function in small laboratory animals with angiocardiopathies.

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任建勋,史雅红,杨斌,郭浩,李磊,孟红旭,刘建勋.大鼠急性心肌缺血损伤后心脏结构和功能改变的超声心动图评价[J].中国比较医学杂志,2019,29(7):17~23.

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  • 收稿日期:2018-10-20
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  • 在线发布日期: 2019-08-01
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