维生素E 对大鼠肾缺血再灌注后心肌损伤的影响
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(1.华北理工大学公共卫生学院预防医学系,河北唐山063210;2.华北理工大学实验动物中心,河北唐山063210;3.华北理工大学基础医学院,河北省慢性疾病重点实验室,河北唐山063210)

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R-33

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Effect of vitamin E on myocardial injury following renal ischemia / reperfusion in rats
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(1.School of Preventive Medicine,North China University of Science and Technology, Tangshan 063200, China.2.Experimental Animal Center, North China University of Science and Technology, Tangshan 063200.3.School of Basic Medical Sciences, North China University of Science and Technology, Hebei Key Laboratory for Chronic Diseases, Tangshan 063000)

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    摘要:

    目的 探讨维生素E(vitamin E, VE)对大鼠肾缺血再灌注(renal ischemia reperfusion, RIR)后心肌损伤的影响?方法 复制大鼠RIR 模型,部分动物再灌注前4 周灌胃给予VE 500 mg/ kg/24 h,观察心肌组织丙二醛(MDA)?髓过氧化物酶(MPO)?黄嘌呤氧化酶(XO)?超氧化物歧化酶(SOD)?一氧化氮(NO)和血浆肌酸激酶(CK)和肌酸激酶同工酶(CK-MB)的变化;测定平均动脉压(MAP)?左心室收缩压(LVSP)?收缩期左室内压上升最大速率(dp / dt max)和舒张期左室内压下降最大速率(-dp / dt max);光镜?免疫组化观察心肌形态和内皮型一氧化氮合酶(e NOS)的表达?Western blot 法检测心肌细胞P47phox 蛋白表达?结果 大鼠RIR 后,心肌组织MDA 含量?MPO 和XO 活性及NO 浓度增加而SOD 活性减少,血浆CK?CK-MB 升高,血流动力学指标下降,镜下心肌细胞水肿?e NOS 阳性细胞数增加,p47phox 蛋白表达增多;给予VE 后,大鼠心肌组织中MDA 含量?MPO 和XO 活性降低而SOD 活性增加,血浆CK?CK-MB 下降,血流动力学指标升高,NO 浓度升高,镜下心肌损伤减轻,e NOS 阳性细胞数增多,p47phox 蛋白表达减少,差异均具有统计学意义?结论 VE 可能通过抗炎?抗氧化?升高NO 含量?减少P47phox 表达等途径对RIR 后的心肌发挥保护作用?

    Abstract:

    Objective To investigate the effect of vitamin E (VE) on myocardium in a rat model of renalischemia reperfusion (RIR). Methods Rats were divided into three groups: a control group, RIR model group, andexperimental (VE + RIR) group fed with VE for 4 weeks before the RIR model was prepared. Contents of malondialdehyde(MDA), myeloperoxidase (MPO), xanthine oxidase (XO), superoxide dismutase (SOD), and nitric oxide (NO) weremeasured in the myocardium. In addition, levels of creatine kinase (CK) and creatine kinase MB isoenzyme (CK-MB) inplasma were determined. Mean arterial pressure (MAP), left ventricular systolic pressure (LVSP), maximal rise rate ofleft ventricular pressure ( dP/ dtmax ) and maximal fall rate of left ventricular pressure (-dP/ dtmax ) were monitored.Myocardial cell morphology and expression of endothelial nitric oxide synthase (eNOS) were observed by light microscopyand immunohistochemistry. Western blot analysis was conducted to quantify P47phox expression in cardiomyocytes. Results Compared with the control group, levels of MDA, MPO, XO, and NO were increased, and the level of SOD wasdecreased in the myocardium of RIR group rats; moreover, CK and CK-MB plasma levels were increased in this group.Compared with the control group, values of MAP, LVSP, dP/ dtmax, and -dP/ dtmax were obviously decreased in the RIRgroup. In addition, aggravation of myocardial damage, number of eNOS-positive cells, and P47phox protein expression wereincreased in the RIR group compared with the control group. Compared with RIR group, the VE + RIR group exhibiteddecreased levels of MDA, MPO, and XO, as well as increased levels of SOD increased in myocardium. In addition, plasmalevels of CK and CK-MB were decreased, NO content was increased, and values of MAP, LVSP, dP/ dtmax, and -dP/ dtmaxwere obviously increased in the VE + RIR group compared with the RIR group. Moreover, compared with the RIR group,microscopic cardiomyocyte injury was reduced, numbers of eNOS-positive cells were increased, and P47phox proteinexpression was decreased in the VE + RIR group. Conclusions VE potentially protects the myocardium from RIR by antiinflammatoryand anti-oxidative mechanisms, as well as by increasing NO content and decreasing P47phox protein expression.

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弭小艺.维生素E 对大鼠肾缺血再灌注后心肌损伤的影响[J].中国比较医学杂志,2019,29(6):65~71.

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  • 收稿日期:2018-11-08
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  • 在线发布日期: 2019-07-16
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