亚甲蓝与七氟醚在肺缺血再灌注(IR)损伤大鼠模型中对血清及肺组织氧化应激指标及炎症因子的影响
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(四川省医学科学院,四川省人民医院麻醉科, 成都 610041)

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R-33

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Effects of methylene blue and sevoflurane on oxidative stress indexes and inflammatory factors in serum and lung tissue in rats with lung ischemia-reperfusion injury
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(Sichuan Academy of Medical Sciences, Department of Anesthesiology of Sichuan People’s Hospital,Chengdu 610041,China)

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    摘要:

    目的 研究亚甲蓝与七氟醚在肺缺血再灌注(ischemia-reperfusion, IR)损伤大鼠模型中对血清及肺组织氧化应激指标及炎症因子影响?方法 60 只雄性SD 大鼠根据随机数字表法分成四组,假手术组(空白对照组)?肺缺血再灌注组(IR 对照组)?亚甲蓝预处理组(MB 组)?七氟醚预处理组(S 组),每组15 只?建立离肺缺血再灌注损伤模型后,比较SD 大鼠采用不同方式处理后,对血清及肺组织中白介素-1β(interleukin-1 β,IL-1β)?白介素-6(IL-6)?肿瘤坏死因子α(tumor necrosis α,TNF-α)等炎性指标和超氧化物歧化酶(SOD)?丙二醛(MDA)?活性氧(ROS)以及三磷酸腺苷(ATP)等氧化应激指标,血液流变学以及肺湿重和干重的影响?结果 MB 组?S 组的肺组织湿/ 干质量比显著高于假手术组,且显著低于IR 组,具有统计学差异( P <0. 05)?MB 组?S 组的湿/ 干质量比组间相比无统计学差异( P >0. 05)?MB 组?S 组的SOD?ATP 水平显著低于假手术组,显著高于IR 组,同时MDA 和ROS 显著高于假手术组,显著低于IR 组,具有统计学差异( P <0. 05),而MB 组?S 组各氧化应激指标组间比较无统计学差异( P >0. 05)?MB 组?S 组肺组织和血清各炎性指标水平显著高于假手术组,显著低于IR 组,具有统计学差异( P <0. 05),MB 组?S 组各炎性指标组间比较无统计学差异( P >0. 05)?MB 组?S 组T1?T2 期的红细胞变形指数?红细胞聚集指数和血浆黏度均低于T0 期,MB 组T1?T2 期各指数均低于S 组,差异有统计学意义( P <0. 05)?结论 亚甲蓝与七氟醚均可改善肺缺血再灌注损伤大鼠中肺组织氧化应激指标,降低血清及肺组织炎症因子水平,改善水肿状态,但亚甲蓝改善血液黏度效果更显著,可有效降低血栓形成风险?

    Abstract:

    Objective To study the effects of methylene blue and sevoflurane on oxidative stress indexes andinflammatory factors in serum and lung tissue in rats with lung ischemia-reperfusion injury. Methods Sixty male Sprague-Dawley rats were divided into four groups according to random number table, sham operation group (blank control group),lung ischemia-reperfusion group ( IR control group), methylene blue pretreatment group ( MB group), sevofluranepretreatment group (S group), 15 in each group. After establishing a model of lung ischemia-reperfusion injury, the ratswere treated respectively, and interleukin-1β (IL-1β) and interleukin-6 (IL-6) were detected in serum and lung tissues.Inflammatory markers such as tumor necrosis factor-α ( TNF-α) and oxidative stress indicators such as superoxidedismutase (SOD), malondialdehyde (MDA), reactive oxygen species (ROS), and adenosine triphosphate (ATP),hemorheology and the effects of lung wet weight and dry weight were assessed. Results The lung wet/ dry mass ratios inthe MB and S groups were significantly higher than that in the sham operation group and significantly lower than that in theIR group ( P < 0. 05). There was no significant difference in wet/ dry weight ratios between the MB group and the S group( P > 0. 05). The levels of SOD and ATP in the MB and S groups were significantly lower than those in the sham operationgroup and significantly higher than those in the IR group, while MDA and ROS were significantly higher than those in thesham operation group and significantly lower than those in the IR group, all showed statistical significance ( P < 0. 05).There was no significant difference in the indexes of oxidative stress in the MB and S groups ( P > 0. 05). The inflammatoryindex levels of lung tissue and serum in the MB and S groups were significantly higher than those in the sham operationgroup and significantly lower than those in the IR group ( P < 0. 05). There was no significant difference in inflammatoryindex levels between the MB and S groups ( P > 0. 05). The erythrocyte deformation index, erythrocyte aggregation index,and plasma viscosity in the MB group and S group at the T1 and T2 phases were lower than those in the T0 phase, andthose in the MB group at the T1 and T2 phases were lower than those in the S group ( P < 0. 05). Conclusions Methyleneblue and sevoflurane can improve the index of oxidative stress in the lung tissue, reduce the level of inflammatory factors inthe serum and lung tissue, and improve the edematous state in IR injury rats. However, methylene blue can decrease the blood viscosity more significantly, which can effectively reduce the risk of thrombosis.

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李佳岑,罗瑶,曾思.亚甲蓝与七氟醚在肺缺血再灌注(IR)损伤大鼠模型中对血清及肺组织氧化应激指标及炎症因子的影响[J].中国比较医学杂志,2019,29(4):88~92.

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  • 收稿日期:2018-09-18
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  • 在线发布日期: 2019-05-10
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