七氟醚对单肺通气致肺损伤保护作用研究
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(1.贵阳中医学院第二附属医院,贵阳 550003; 2. 贵州医科大学附属医院,贵阳 550004)

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R-33

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Protective effects of sevoflurane on lung injury induced by one-lung ventilation in rabbits
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(1. The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550003, China.2.Affiliated Hospital of Guizhou Medical University, Guiyang 550004)

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    摘要:

    目的 探讨七氟醚对单肺通气(one-lung ventilation, OLV)所致兔急性肺损伤后的保护作用及机制?方法 采用36 只白兔随机分为3 组,分别为假手术组?单肺通气组?单肺通气+七氟醚组?其中单肺通气+七氟醚组按七氟醚浓度不同分为几个亚组?采用ELISA 法检测兔肺组织中花生四烯酸(arachidonic acid, AA)含量,同时测定肺W/ D 值,通过HE 染色观察肺组织形态学变化;采用RT-qPCR 法检测肺组织中Clara 细胞分泌蛋白(CCSP)和胞质型磷脂酶A2(C-PLA2)mRNA 表达水平;采用Western blot 法检测肺组织中CCSP 和C-PLA2 蛋白表达?结果 单肺通气组肺组织中花生四烯酸含量?肺损伤程度及C-PLA2 蛋白和mRNA 表达水平均较假手术组明显增加( P <0. 05),而CCSP 蛋白和mRNA 表达水平明显降低( P <0. 05);单肺通气+七氟醚组兔肺组织中花生四烯酸?肺损伤及肺组织C-PLA2 蛋白和mRNA 表达水平较单肺通气组下降( P <0. 05),而CCSP 蛋白和mRNA 表达增加;单肺通气+不同浓度七氟醚组肺组织中花生四烯酸含量?肺损伤程度及C-PLA2 蛋白和mRNA 表达水平随浓度的增加而逐渐降低( P <0. 05),而CCSP 蛋白及mRNA 表达无显著性差异?结论 七氟醚保护单肺通气所致肺损伤的作用机制可能与下调C-PLA2 途径,减少肺组织中AA 生成有关?

    Abstract:

    Objective To study the protective effect and mechanism of action of sevoflurane on the acute lunginjury in rabbits with one-lung ventilation. Methods Thirty-six rabbits were randomly divided into three groups: shamoperationgroup, one-lung ventilation group, and one-lung ventilation + sevoflurane group. The sevoflurane concentration inthe one-lung ventilation + sevoflurane group was varied to create several subgroups. The level of arachidonic acid in lungtissue of rabbits was determined by ELISA, and the W/ D value of lung was measured. The morphological changes of lungtissue were examined pathology. The expression levels of Clara cell secretory protein ( CCSP ) and cytoplasmicphospholipase A2 (C-PLA2) mRNA in the lung tissue were determined by RT-qPCR. The expression levels of CCSP andC-PLA2 protein in lung tissue were detected by western blotting. Results The level of arachidonic acid, the degree of lunginjury, and the expression of C-PLA2 protein and mRNA in the lung tissue of the one-lung ventilation group weresignificantly higher than those of the sham operation group ( P < 0. 05), while the expression levels of CCSP protein andmRNA were significantly lower ( P < 0. 05). Arachidonic acid, lung injury, C-PLA2 protein, and mRNA expression in thelung tissue of rabbits in the one-lung ventilation + sevoflurane group were lower than those in the one-lung ventilation group( P < 0. 05), while CCSP protein and mRNA expression levels were increased. The level of arachidonic acid, the degree oflung injury, and the expression of C-PLA2 protein and mRNA in lung tissue of the one-lung ventilation + sevoflurane groupwere decreased with increasing sevoflurane concentration ( P < 0. 05), but there were no significant differences in theexpression of CCSP protein and mRNA. Conclusions The protective mechanism of sevoflurane against lung injury inducedby one-lung ventilation may be related to downregulation of the C-PLA2 pathway and reduction of arachidonic acid production in the lung tissue.

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种朋贵,丁瑜,高鸿.七氟醚对单肺通气致肺损伤保护作用研究[J].中国比较医学杂志,2019,29(4):82~87.

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  • 收稿日期:2018-09-17
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  • 在线发布日期: 2019-05-10
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