胶原诱导性关节炎病/ 证模型大鼠血清细胞因子表达谱的初步研究
作者:
作者单位:

(1.北京中医药大学中药学院,北京 102488; 2.北京中医药大学东方医院,北京 100078)

作者简介:

通讯作者:

中图分类号:

R-33

基金项目:


Preliminary study on serum cytokine expression profiles in rats with collagen-induced arthritis, cold-damp arthralgia or damp-heat arthralgia
Author:
Affiliation:

(1. School of Chinese Materia Medica, Beijing University of Chinese Medicine,Beijing 102488, China.2. Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 研究胶原诱导性类风湿关节炎?寒湿痹和湿热痹病/ 证模型大鼠血清细胞因子的表达?方法 采用牛Ⅱ型胶原与弗氏完全佐剂复制类风湿关节炎(rheumatoid arthritis, RA)模型,在此基础上复加寒湿?湿热刺激建立寒湿痹及湿热痹病证结合模型,用微阵列抗体芯片检测血清细胞因子的表达?结果 与正常组比较,RA 组activin A?CNTF?IFN-γ?TNF-α 上调,MIP-3α?MMP-8 下调;寒湿痹组CINC-2α?GM-CSF?IFN-γ 上调,MIP-3α 下调;湿热痹组ICAM-1 上调,IFN-γ?MIP-3α?RAGE 下调?与RA 组比较,寒湿痹组β-NGF?CINC-2α?MMP-8?PDGF-AA 上调, activin?TNF-α 下调;湿热痹组MMP-8 上调, activin?CNTF?IFN-γ?IL-1α?IL-6?MIP-3α?RAGE?TNF-α 下调?寒湿痹组较与RA 热痹湿热痹组比较,CINC-2α?IFN-γ?IL-1α?MIP-3α?PDGF-AA?RAGE 均上调?与正常组比较,RA组及寒湿痹组IFN-γ/ IL-4 比值升高,湿热痹组比值明显降低;与RA 组比较,寒湿痹组比值升高,湿热痹组比值明显下降?结论 病/ 证因素对大鼠血清细胞因子表达谱有明显影响,且各模型间存在明显差异?

    Abstract:

    Objective To determine the serum cytokine levels in collagen-induced rheumatoid arthritis (RA)rats, cold-damp arthralgia rats, and damp-heat arthralgia rats by using antibody arrays. Methods A rheumatoid arthritismodel was established by intracutaneous injection of type II collagen and complete Freund’ s adjuvant (CFA). On thisbasis, cold-damp or damp-heat stimulation was applied to the RA rats in order to replicate cold-damp arthralgia or dampheatdamp-heat arthralgia. Serum cytokine levels in the rats were determined using antibody arrays. Results Comparedwith the normal group, the activin, CNTF, IFN-γ, and TNF-α levels were upregulated, while MIP-3α and MMP-8 weredownregulated in the RA group. The CINC-2α, GM-CSF, and IFN-γ levels were upregulated and MIP-3α wasdownregulated in the cold-damp arthralgia group. ICAM-1 was upregulated, while IFN-γ, MIP-3α, and RAGE weredownregulated in the damp-heat arthralgia group. Compared with those in the RA group, β-NGF, CINC-2α, MMP-8, andPDGF-AA were upregulated, while activin A and TNF-α were downregulated in the cold-damp arthralgia group. MMP-8 wasupregulated, and activin A, CNTF, IFN-γ, IL-1α, IL-6, MIP-3α, RAGE, and TNF-α were downregulated in the dampheatarthralgia group. Compared with the damp-heat arthralgia group, CINC-2α, IFN-γ, IL-1α, MIP-3α, PDGF-AA, andRAGE were upregulated in the cold-damp arthralgia group. Compared with that in the normal group, the ratio of IFN-γ/ IL-4was increased in the RA group and cold-damp arthralgia group, but was decreased significantly in the damp-heat arthralgiagroup. Compared with that in the RA group, the ratio increased in the cold-damp arthralgia group, and decreasedsignificantly in the damp-heat arthralgia group. Conclusions Diseases (RA) and syndromes (cold-damp or damp-heat)have a significant influence on serum cytokine expression profiles in rats, and there are clear differences among different models.

    参考文献
    相似文献
    引证文献
引用本文

丁子桐,熊海霞,高琴琴,靖卫霞,袁芳,侯秀娟,朱跃兰,孙文燕.胶原诱导性关节炎病/ 证模型大鼠血清细胞因子表达谱的初步研究[J].中国比较医学杂志,2019,29(4):28~33.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2018-09-14
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2019-05-10
  • 出版日期:
自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
关闭