参麦对急性脑梗死小鼠脑组织的保护作用及相关机制的探讨
作者:
作者单位:

(1. 南华大学附属第一医院神经内科;2.南华大学附属南华医院麻醉科;3. 南华大学附属第一医院重症医学科,湖南衡阳 421000)

作者简介:

通讯作者:

中图分类号:

R-33

基金项目:


Exploration of the protective effect of Shenmai injection on brain tissue and its related mechanisms in a mouse model of cerebral infarction
Author:
Affiliation:

(1. Department of Neurology, the First Affiliated Hospital of University of South China; 2. Department of Anesthesiology,Nanhua Hospital of University of South China; 3. Department of Critical Care Medicine, The First Affiliated Hospital of University of South China, Hengyang,421000, China)

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 研究参麦注射液对急性脑梗死小鼠脑组织保护作用并初步探讨其可能的机制?方法 本实验以30 只成年雄性CD-1 小鼠为研究对象,随机分为对照组?模型组和实验组,每组各10 只?采用线栓法构建急性脑梗死小鼠模型,实验组给予0. 3 mL 参麦注射液治疗,对照组和模型组均给予同等剂量生理盐水干预,比较各组小鼠的红细胞功能指标?采用TUNEL 法测定脑组织的神经元凋亡率,使用Western blot 和q-PCR 技术检测小鼠脑皮层calpain-1 及Bcl-2 的表达情况?结果 与对照组比较,实验组RBC-C3bR 明显降低,RBC-ICR 显著升高( P <0. 05)?与模型组比较,实验组RBC-C3bR 明显增高,RBC-ICR 显著降低( P <0. 05)?模型组和实验组小鼠脑组织切片的TUNEL 阳性细胞数显著高于对照组( P <0. 05);实验组小鼠脑组织切片的TUNEL 阳性细胞数明显低于模型组小鼠( P <0. 05)?与对照组小鼠比较,模型组和实验组小鼠的calpain-1 蛋白和mRNA 表达水平显著升高,Bcl-2蛋白和mRNA 表达水平显著降低( P <0. 05);与模型组小鼠相比,实验组小鼠calpain-1 蛋白和mRNA 表达水平显著降低,Bcl-2 蛋白和mRNA 表达水平显著升高( P <0. 05)?结论 参麦注射液可改善急性脑梗死小鼠红细胞功能和抑制神经元凋亡,其可能的机制是下调calpain-1 蛋白的表达,上调抗凋亡蛋白Bcl-2 的表达,发挥脑组织神经保护作用?

    Abstract:

    Objective To study the protective effect of a Chinese medicine,Shenmai injection, on brain tissue in a mouse model of cerebral ischemia and explore its possible mechanism. Methods Thirty healthy adult male CD-1 micewere randomly divided into control, model, and experimental groups with 10 mice in each group. The mouse model of acutecerebral infarction was established by permanent middle cerebral artery occlusion. The experimental group was treated withShenmai injection. The control and model groups were administered the same dose of physiological saline. The red blood cellfunctional indexes were compared among groups. The neuonal apoptosis rate of brain tissue was determined by TUNELassay. Calpain-1 and Bcl-2 expression in the mouse cortex was detected by Western blot and qPCR. Results Comparedwith the control group, RBC-C3bR was significantly decreased and RBC-ICR was significantly increased in the experimentalgroup ( P < 0. 05). Compared with the model group, RBC-C3bR was significantly increased, and RBC-ICR was decreasedsignificantly in the experimental group( P < 0. 05). The number of TUNEL-positive cells in the brain tissue sections ofmodel and experimental groups was significantly higher than that of the control group ( P < 0. 05). The number of TUNELpositivecells in brain tissue sections of the experimental group was significantly lower than that of the model group ( P <0. 05). Compared with the control group, the expression levels of calpain-1 protein and mRNA in model and experimentalgroups were significantly increased, and the expression levels of Bcl-2 protein and mRNA were decreased significantly ( P <0. 05). Compared with the experimental group, the expression levels of calpain-1 protein and mRNA were significantlydecreased, and that of Bcl-2 protein and mRNA were increased significantly ( P < 0. 05). Conclusions Shenmai injectionimproves erythrocyte functions and inhibites neuronal apoptosis in this mouse model of cerebral infarction. The possiblemechanism of the protective effect of Shenmai injection on brain infarction may be the downregulating of expression of calpain-1 protein and upregulating of expression of anti-apoptotic protein Bcl-2 in the brain tissues.

    参考文献
    相似文献
    引证文献
引用本文

阮中繁,谢明,李艳,王桥生.参麦对急性脑梗死小鼠脑组织的保护作用及相关机制的探讨[J].中国比较医学杂志,2019,29(4):23~27.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2018-10-31
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2019-05-10
  • 出版日期:
自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
关闭