Objective To explore the effect of intermedin (IMD) and adrenomedullin (ADM) on cerebral microcirculation in rats with cerebral ischemia. Methods Rat cerebral ischemia (CI) model was established by middle cerebral artery occlusion. 40 SPF male adult Sprague-Dawley (SD) rats were randomly divided into three groups:CI+NS (normal saline) group, CI+ADM group and CI+IMD group, which were used to observe the changes of brain surface microcirculatory perfusion with a laser Doppler flowmeter. Results The differences of brain surface microcirculatory perfusion were statistically significant among the CI+NS group, CI+ADM group and CI+IMD group (F=53.426, P< 0.05). Multiple comparison showed that the brain surface microcirculatory perfusion in the CI+IMD group was higher than that of the CI+NS group and CI+ADM group. Conclusions Intermedin can improve the cerebral microcirculation in rats with cerebral ischemia, and its therapeutic effect is better than adrenomedullin.