高热大鼠不同程度寒颤模型的构建
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福建省科学技术厅2014年福建省科技重点项目(2014I0002)。


Establishment of rat models of fever and shivering in different degrees
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    摘要:

    目的 探索高热大鼠降温处理过程中发生寒颤的体温阈值,构建不同程度寒颤大鼠模型。方法 使用20%干酵母混悬液将体重为(200±20) g、基础体温为36.8℃~38.3℃的雄性SD大鼠致热,选取致热成功的大鼠40只,随机分为4组,每组10只,分别使用10、20、40 mL冰袋对高热大鼠施行颈部和腋下探索性降温处理30 min,对照组不进行降温。观察大鼠寒颤的表现、监测大鼠发生寒颤的肛温阈值。结果 高热大鼠在降温处理30 min内,对照组和10 mL冰袋组大鼠身体任一部位均未发生寒颤;20 mL冰袋组大鼠发生轻度寒颤,表现为竖毛,头、颈部颤抖,伴或不伴上肢颤抖,发生轻度寒颤的平均肛温阈值为37.25℃,轻度寒颤发生率为100%;40 mL冰袋组大鼠发生重度寒颤,表现为竖毛,头、颈部、四肢和躯干均剧烈颤抖,尾巴肌张力增强,发生重度寒颤的平均肛温阈值为37.07℃,重度寒颤发生率为90%。结论 对高热大鼠进行冰袋降温干预可以构建出较为理想的无寒颤、轻度寒颤、重度寒颤模型。

    Abstract:

    Objective To explore the body temperature thresholds of shivering in febrile rats during therapeutic hypothermia, and establish rat models of shivering in different degrees. Methods A randomized controlled trial was adopted. Rats weighted 200±20 g and basal body temperature of 36.8-38.3℃ were induced with fever using 20% dry yeast solution through dorsal subcutaneous injection. 40 rats were randomly divided into 4 groups:10 mL ice pack group, 20 mL ice pack group, 40 mL ice pack group and control group, 10 rats in each group. Physical cooling was implemented for 30 minutes in the neck and armpits. No cooling measures was given to the control group. The shivering of rats were observed, and the threshold of anal temperature was monitored. Results In the rats with hyperthermia, shivering was not observed in any part of the rats in the control group and in the therapeutic hypothermia group of 10 mL ice pack. The rats in the therapeutic hypothermia group of 20 mL ice pack developed mild shivering, which manifested as piloerection, head and neck trembling, with or without upper limbs trembling. The threshold of the average rectal temperature of mild shivering was 37.25℃, The incidence of mild shivering was 100%. The rats in the therapeutic hypothermia group of 40 mL ice pack developed severe shivering, which manifested as piloercetion, head, neck, limbs and trunk were trembling, and tail muscle tension increased. The threshold of the average rectal temperature of severe shivering was 37.07℃, severe shivering occurred in 90% of the rats. Conclusions No shivering, mild shivering, and severe shivering models can be established by intervention with ice pack in rats with high fever during therapeutic hypothermia.

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吴冬梅,郑翠红,吴冠虹,李华萍.高热大鼠不同程度寒颤模型的构建[J].中国比较医学杂志,2017,27(10):51~54.

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  • 收稿日期:2017-02-17
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  • 在线发布日期: 2017-10-23
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