腹壁撤退反射实验测量方法概述
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国家自然科学基金(81503665)。


A summary of measurement methods for abdominal withdrawal reflex test
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    摘要:

    腹壁撤退反射(abdominal withdrawal reflex,AWR)实验,通过扩张肠道来评价动物的行为学反应,是目前应用最广的评价动物内脏敏感性的方法。然而,AWR实验操作涉及因素多,目前鲜有较为公认的操作规程。本文通过对涉及AWR实验测量方法的文献进行梳理,归纳出AWR操作的基本步骤,总结不同测量方法在扩张方式、气囊种类等相关因素方面的差异,并对相关因素的具体操作提出建议,以期为后续使用该方法评价动物内脏敏感性的研究者提供选择方法,为实验操作的标准化方案制定奠定必要基础。

    Abstract:

    Abdominal withdrawal reflex (AWR) test, which evaluates the behavioral response of animals through expanding the intestinal tract, is the most widely used method to assess animal's visceral sensitivity in recent years. However, as AWR experimental operation involves many factors, it's hard to find a commonly recognized operating procedure. Through review of literature of AWR experimental measuring methods, this article summarizes the basic steps of AWR operation. Meanwhile, it scrutinizes the differences of various measuring methods in relevant factors, including expansion method and airbag method, with special operating suggestions to them. Hopefully, this article will provide an option to future researchers who will use this method to evaluate animal's visceral sensitivity, and provide a necessary foundation for the standardization of the experimental operation.

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陈颖,赵妍,罗丹妮,黄辰,周思远.腹壁撤退反射实验测量方法概述[J].中国比较医学杂志,2017,27(8):89~93.

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  • 收稿日期:2017-03-17
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  • 在线发布日期: 2017-08-31
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