Abstract：【Objective】 A sterile golden hamster model was established by cesarean section purification method.【Methods】The donor female golden hamsterrats selected SPF grade golden hamster, male and female were mated 1:1, and were separated from male rats after meeting the peg. The cage time of the surrogate mothers rats should be one week earlier than that of the donor mothers. The pregnant parturient golden hamsters underwent hysterectomy in a super-clean workbench. at the time of delivery, andThe uterus were transferred into isolation kits for purification. The uterus wasand stripped to obtain the milk mice to be purifiedfor purification, . and sterile Sterile ICR mice and sterile SD rats were respectively used for milk replacement, and transferred to isolation kits for feeding after successful milk separation. The sterility status of feeding isolation kits is tested monthly. 【Results】Three caesarean sections were performed, but the first and second lactation failed. The third milk replacement was successful, and 18 young rats hamsters were obtained with survival rate of 88% and survival rate of 66% after milk release. All of them hamsters were qualified according totested by GB /T 14926.41-2001.【Conclusion】By cesarean section purification technique, sterile ICR mice and sterile SD rats were used for milk replacement to achieve the purpose of microbial purification, and the sterile golden hamster model was obtained.