1.个人;2.Jiangxi University of Chinese Medicine;3.Jiangxi University of Traditiona Medicine;4.Jinggangshan Mujiangyeke Augriculuture and Forestry Development Co,Ltd
摘要：目的 探究复方木姜叶柯活性茶对高尿酸血症肾病小鼠的尿酸和肾功能的影响，为开发治疗高尿酸血症肾病药物和功能性食品提供实验依据。方法 采用氧嗪酸钾和腺嘌呤建立高尿酸血症肾病小鼠模型。小鼠随机分为正常组、模型组、苯溴马隆阳性药组(10 mg?kg-1?d-1)、复方木姜叶柯活性茶高、中、低剂量组（10g?kg-1?d-1、3.33g?kg-1?d-1、1.11g?kg-1?d-1）。末次给药1h后，取尿液，采用CBB法测定尿蛋白（UP），脲酶法检测尿素氮（UUN）；眼眶取血，采用酶比色法检测血清中尿酸（UA）含量，脲酶法检测血清中尿素氮（BUN）含量，酶联免疫法检测血清中白介素细胞6（IL-6）、肿瘤坏死因子（TNF-α）含量；荧光定量分析法检测小鼠肾脏中尿酸盐转运蛋白1（URAT1）及葡萄糖转运体9（GLUT9）含量；取肾脏组织，HE染色观察组织病理改变情况。结果 与正常组相比，模型组小鼠UP、UUN、UA、BUN、IL-6、URAT1及GLUT9水平显著增高（P＜0.01），TNF-α水平增高（P＜0.05），肾组织结构正常。与模型组相比，苯溴马隆阳性药组中UP、UUN、UA、BUN含量显著降低（P＜0.01），IL-6及TNF-α水平降低（P＜0.05），URAT1水平增加，肾脏有少量肾小球萎缩变形，肾偶见肾小管扩张，无炎性细胞浸润。与模型组相比，复方木姜叶柯活性茶高剂量组中UP、UUN、UA、BUN、IL-6及URAT1水平显著降低（P＜0.01），TNF-α与GLUT9水平降低（P＜0.05）；中剂量组中UP、UA、UUN含量及IL-6、URAT1、GLUT9水平显著降低（P＜0.01），BUN与TNF-α水平降低（P＜0.05）；低剂量组中UP、UUN、UA含量及IL-6、URAT1水平显著降低（P＜0.01），BUN含量及 TNF-α、GLUT9水平降低（P＜0.05）；复方木姜叶柯活性茶组小鼠肾脏情况均有所改善。结论：复方木姜叶柯活性茶能降低高尿酸血症肾病小鼠的尿酸，对肾脏具有一定的保护作用，其机制可能与抑制尿酸重吸收有关，具体机制有待于进一步研究。
Abstract：Objective To explore the effect of compound active tea of Lithocarpus litseifolius on uric acid and kidney function on mice with hyperuricemia nephropathy , and to provide experimental basis for the development of hyperuricemia nephropathy drugs and functional food. Methods The mouse model of hyperuricemia nephropathy was established by potassium oxyoxide with adenine. Mice were randomly divided into normal group, model group, benbromarone positive drug group (10 mg?kg-1?d-1), and Compound active tea of Lithocarpus litseifolius high,middle, low-dose groups (10g?kg-1?d-1,3.33g?kg-1?d-1,1.11g?kg-1?d-1).1h after the last dose, urine protein (UP) was measured by CBB method,urea nitrogen (UUN) was measured by the urease method;Blood was collected for uric acid (UA) by enzyme ratio method and urea nitrogen (BUN) by urease method;the serum content of interleukin cells 6 (IL-6) and tumor necrosis factor(TNF-α) was measured byeuzymelinked immunosorbent assay;uate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) in mouse kidneys were measured by quantitative fluorescence analysis.Kidney tissues were harvested and histopathological changes were observed by HE staining.Results Compared with the normal group,the UP, UUN, UA, BUN, IL,URAT1 and ULUT9 of model group mice were significant increased (P<0.01),TNF-α was increased (P<0.05),the renal tissue structure was normal.Compared with the model group,the UP,UUN,UA,BUN of benbromarone positive drug group were significant decreased(P<0.01),IL-6 and TNF-α were decreased(P<0.05),URAT1 was increased(P<0.05),little glomerular atrophy and deformation in the kidney,kidney tubular dilatation was occasionally seen,and no inflammatory cell infiltration.Compared with the model group,the UP,UUN,UA,BUN,IL-6 and URAT1 of high-dose group of compound active tea of Lithocarpus litseifolius were significant decreased(P<0.01),TNF-α and GLUT9 levels were decreased(P <0.05);the UP, UA, UUN content , the levels of IL-6, URAT 1and GLUT9 of middle-dose group of compound active tea of Lithocarpus litseifolius were significant decreased(P<0.01),BUN together with reduced levels of TNF-α(P <0.05);the UP,UUN,UA,IL-6 and URAT1 of low-dose group of compound active tea of Lithocarpus litseifoliuswere significant decreased(P<0.01),BUN content and reduction of TNF-α and GLUT9 levels (P<0.05),the kidney condition of the mice in compound active tea of Lithocarpus litseifolius group was improved.Conclusion: the compound active tea of Lithocarpus litseifolius can reduce uric acid in mice with hyperuricemia nephropathy, and it has a certain protective effect on the kidney,the mechanism may be related to the inhibition of uric acid reabsorption, and the specific mechanism should be further investigated.