Zhejiang Pharmaceutical University
【摘要】 目的 建立有效稳定的小鼠慢性酒精中毒性脑损伤模型。 方法 将C57BL/6J小鼠随机分成对照组和模型组，模型组小鼠除基础饮用水中添加5%(v/v)酒精外，同时灌胃28%(v/v)酒精，灌胃剂量前两周呈梯度增长（从0逐渐增加至6 g/kg体重），随后四周维持在6 g/kg体重，对照组饮用水中不添加酒精同时灌胃等量生理盐水。实验结束后，通过行为学实验测定小鼠的认知功能和运动能力，通过组织病理染色检测小鼠脑组织的形态学变化。 结果 与对照组比较，模型组小鼠在行为学实验中表现出认知功能和运动功能障碍，并且，模型组小鼠脑组织病理染色显示海马区形态损伤和细胞坏死。 结论 实验成功有效建立了小鼠慢性酒精中毒性脑损伤模型，该模型可应用于慢性酒精中毒性脑病的机制和治疗药物研究。
【Abstract】 Objective To establish an effective and reliable chronic alcoholic brain injury model in mice. Methods C57BL/6J mice were randomly assigned into the control group and the model group. Mice in the model group were given free access to 5% (v/v) alcohol in drinking water, and were intragastrically administered with 28% (v/v) alcohol. The gavage dosage increased gradually over the first two weeks (from 0 to 6 g/kg body weight), and remained at 6 g/kg body weight for the subsequent four weeks. Mice in the control group were provided with normal water and given same amount of saline via gavage. At the end of the experiment, the cognitive function and motor ability of mice were evaluated through behavioral tests. The morphological changes of brain tissue of mice were examined by histopathological staining. Results Compared to the control group, mice in the model group showed cognitive impairments and motor dysfunction in the behavioral tests. The pathological examination of the brain tissue from the model group mice showed morphological damage and cell necrosis in the hippocampus. Conclusions The mouse model of chronic alcoholic brain injury was successfully and effectively established in this study, providing a valuable tool for investigating the underlying mechanisms and potential therapeutic interventions for chronic alcoholic encephalopathy.