Henan University of Chinese Medicine
目的 总结骨肉瘤(Osteosarcoma，OS)动物模型造模要点，为完善其造模方法和评价指标提供参考与建议。方法 通过检索中国知网、万方数据库、PubMed中OS动物模型相关文献，建立数据库，总结其实验动物种类、性别、造模方法、癌细胞株种类、检测指标等，建立数据库进行统计分析。结果 共纳入284篇文献，统计分析发现：OS模型动物首选BALB/c-nu/nu裸鼠（227例，75.17%），其次是SD大鼠（20例，6.62%）。造模方式多选用背部皮下细胞液移植法（66例，21.85%）、腋部皮下细胞液移植法（55例，18.21%）或原位细胞液移植法（51例，16.89%）等；癌细胞株种类以人源MG-63细胞（100例，33.11%），鼠源UMR-106细胞（39例，12.91%）为主；检测指标主要选择肿瘤组织表观指标（238例，83.80%）、肿瘤组织HE染色（129例，45.42%）、动物表观指标（94例，33.10%）、肿瘤组织免疫组化（89例，31.34%）。结论 目前OS模型多选用4~6周龄BALB/c-nu/nu裸鼠作为实验动物，采用人源MG-63细胞异位移植法（背部、腋下移植）建立OS动物模型，模型检测指标以动物表观指标、肿瘤表观指标、肿瘤组织病理进行整体评价。但目前依旧缺少与临床吻合度高的动物模型制备及评价标准，本文通过文献挖掘、数据分析总结其优缺点，以期为建立良好的OS模型提供参考，更好地应用于OS机制研究及新药开发。
Objective To summarize the key points of animal modeling of osteosarcoma, and to provide reference and suggestions for improving the modeling methods and evaluation indexes.Methods The database was established by searching the relevant literature on the animal model of osteosarcoma in CNKI, Wanfang database and PubMed. The species of experimental animals, gender, modeling methods, types of cancer cell lines and detection indicators were summarized, and the database was established for statistical analysis. Results A total of 284 literatures were included. Statistical analysis found that BALB / c-nu / nu nude mice were preferred in osteosarcoma model animals(227 cases，75.17%), followed by SD rats (20 cases, 6.62%). Subcutaneous cell fluid transplantation in the back (66 cases, 21.85%), subcutaneous cell fluid transplantation in the axils (55 cases, 18.21%) or in situ cell fluid transplantation (51 cases, 16.89%) were used as modeling methods. Human MG-63 cells (100 cases, 33.11%) and mouse UMR-106 cells (39 cases, 12.91%) were selected as the cancer cell lines.The most detected indexes were tumor tissue apparent index (238 cases, 83.80%), tumor tissue HE staining (129 cases, 45.42%), Animal apparent indicator (94 cases, 33.10%)，tumor tissue immunohistochemistry (89 cases, 31.34%), etc. Conclusions At present, osteosarcoma BALB / c-nu / nu nude mice aged 4 to 6 weeks are used as experimental animals, and human MG-63 cell heterotopic transplantation (back and axillary transplantation) is used to establish the animal model, and the detection indexes of osteosarcoma are comprehensively evaluated by animal apparent index, tumor apparent index and tumor histopathology. It is suggested to select serum biochemical index, apparent index of tumor tissue as well, HE staining of tumor tissue and immunohistochemistry of tumor tissue to evaluate the model. However, there is still a lack of animal model preparation and evaluation criteria with high clinical consistency. In this paper, the advantages and disadvantages are summarized through literature mining and data analysis, in order to provide reference for the establishment of a good OS model and better application to OS mechanism research and new drug development.