1.Shanghai Institute for Biomedical Pharmaceutical Technologies;2.Shanghai Institute for Biomedical Pharmaceutical Technologies
目的 明确低剂量BPA和DEHP对成年大鼠前列腺AKR1C3的影响。 方法 56只成年雄性SD大鼠随机分成7组，每组8只，分别灌胃给予BPA（10.0µg/kg、30.0µg/kg和90.0 µg/kg），DEHP（30.0µg/kg 、90.0µg/kg 和270.0 µg/kg）和溶媒，连续4周。动物于末次给药24 h后，麻醉后采血，剖取前列腺并分叶，利用ELISA法检测血清和前列腺中AKR1C3水平，利用免疫组化法分析各叶前列腺中AKR1C3的表达情况。结果 给予BPA后，腹侧前列腺90µg/kg剂量组AKR1C3表达显著性高于对照组（P<0.05）；背侧前列腺10µg/kg剂量组AKR1C3水平和蛋白表达均显著性高于对照组（P<0.01，P<0.001）。给予DEHP后，270µg/kg剂量组血清AKR1C3水平显著性高于对照组（P< 0.001），腹侧前列腺各组AKR1C3水平均显著性高于对照组（P< 0.05，P<0.01），270µg/kg剂量组AKR1C3蛋白表达显著性高于对照组（P<0.05）；背侧前列腺90µg/kg 和270µg/kg剂量组AKR1C3表达显著性高于对照组（P<0.001，P<0.05）。结论 低剂量BPA和DEHP均能促进成年大鼠前列腺AKR1C3表达，但各叶前列腺腹对BPA和DEHP的敏感度有所不同。
Objective To investigate the effects of of low dose of bisphenol A (BPA) and di (2-ethyl) hexyl phthalate (DEHP) on Aldo-keto reductase 1C3 (AKR1C3) in adult SD rats. Method 56 adult male SD rats were randomly divided into 7groups (8 rats in each group) and they were given BPA (10.0µg/kg,30.0µg/kg,90µg/kg, i.g.), DEHP (30.0µg/kg ,90.0µg/kg ,270 µg/kg, i.g.) and vehicle respectively once a day for 4 weeks. The animals were sacrificed on the day subsequent to last treatment and the blood was collected, the prostate tissues were dissected and categorized into different lobes. The levels of AKR1C3 in serum and prostate were detected by enzyme-linked immunosorbent assay (ELISA), and the expression of AKR1C3 in each lobe of prostate was analyzed by immunohistochemistry. Result After the administration of BPA, the expression of AKR1C3 in 90µg/kg and 270µg/kg group increased, and there was significant difference in the high-dose group (P<0.05); The level and protein expression of AKR1C3 in dorsal prostate increased, and there was significant difference in 10µg/kg group (P<0.01, P<0.001). After administration of DEHP, the level of serum AKR1C3 in 270µg/kg group was significantly higher than that in the control group (P<0.001), the level of AKR1C3 in the ventral prostate was significantly higher than that in the control group (P<0.05, P<0.01), and the expression of AKR1C3 protein increased, there was significant difference in 270µg/kg group (P<0.05); The level of AKR1C3 in 90µg/kg and 270µg/kg dose group of dorsal prostate was higher than that in the control group, and there was significant difference in 30µg/kg group (P<0.05). The expression of AKR1C3 protein in each group increased, and there was significant difference in 30µg/kg and 90µg/kg dose group (P<0.001, P<0.05). Conclusion Low-dose BPA and DEHP can promote the expression of AKR1C3 in the prostate of adult SD rats, but the sensitivity of ventral and dorsal lobes of prostate to BPA and DEHP is different.