基于 NLRP3 研究放射对小鼠涎腺组织损伤的影响
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1.遵义医科大学附属口腔医院,贵州 遵义 563000;2.邵阳市中心医院口腔科,湖南 邵阳 422000;3.遵义医科大学第五附属医院,广东 珠海 519000

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31

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R-33

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Effect of radiation on damage of mouse salivary gland tissue based on NLRP3
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1. Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi 563000, China.2. Department of Stomatology, the Central Hospital of Shaoyang, Shaoyang 422000.3. the Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai 519000

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    摘要:

    目的 研究放射对小鼠涎腺组织形态、功能及 NLRP3 表达的影响,为修复放射性涎腺组织损伤提供新思路。 方法 建立小鼠下颌下腺放射损伤模型,记录饮水情况,于照射后 1、3、7、14 d 进行唾液流率检测,HE染色观察下颌下腺损伤情况,免疫组织化学法及 Real-time PCR 检测 NLRP3 及 Caspase-1 在小鼠放射性下颌下腺损伤中的表达情况。 结果 随着时间的累积,放射组小鼠饮水量逐渐增多,唾液流率减少,下颌下腺中炎症细胞不断增多,腺泡细胞逐渐出现核固缩及空泡化等病变;在照射后的 7 和 14 d,放射组 NLRP3 和 Caspase-1 蛋白及基因表达水平均明显高于正常组(P<0. 05)。 结论 辐射可诱导小鼠下颌下腺组织损伤,并激活 NLRP3 炎症体促使其表达量增加。

    Abstract:

    Objective To study the effects of radiation on the morphology, function, and NLRP3 expression of mouse salivary gland tissue, and provide new ideas to repair radiation-induced damage to salivary gland tissue. Methods We established a mouse model of radiation-induced submandibular gland injury and Recorded the weight of drinking water.The salivary flow rate was assessed, and HE staining was used to observe submandibular gland injury.Immunohistochemistry and Real-time PCR were used to assess expression of NLRP3 and Caspase-1 in the radiation-injured submandibular gland of mice at 1, 3, 7 and 14 days after radiation exposure. Results Over time, the amount of water consumed by radiation group mice was gradually increased, the salivary flow rate was decreased, and inflammatory cells in the submandibular gland continued to increase. Acinar cells gradually showed lesions, such as nuclear pyknosis and vacuolization. At 7 and 14 days after radiation exposure, expression levels of NLRP3 and Caspase-1 proteins and genes in the radiation group were significantly higher than those in the normal group (P<0. 05). Conclusions Radiation damages mouse submandibular gland tissue and activates the NLRP3 inflammasome to increase its expression level.

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吴玉琪,黄桂林,肖丽君,张 敏,张霓霓.基于 NLRP3 研究放射对小鼠涎腺组织损伤的影响[J].中国比较医学杂志,2024,34(5):~26.

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  • 收稿日期:2023-08-25
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  • 在线发布日期: 2024-06-26
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