Objective To investigate the factors affecting the establishment of a mouse model of alcoholic liver injury. Methods A total of 150 specific-pathogen-free male ICR mice were divided into three groups to study the possible influences of different experimental conditions, including modeling period, frequency, type, and dosage of modeling agent, and time interval between doses. Mice in the three groups received 60% ethanol and alcohol with 53% volume ratio by gavage at different doses, frequencies, and time intervals and the mortality rates were calculated. We also detected alanine aminotransferase (ALT) and aspartate transaminase (AST) serum levels and malondialdehyde (MDA), glutathione(GSH), and triglyceride (TG) levels in liver tissue homogenate. Liver pathology was examined in tissue sections. Results The mortality rates in the 1-day modeling and double-dose liquor modeling groups were 20% and 40%, respectively, and the mortality rates in the 4, 6, 8, and 12 h interval dosing groups were 40%, 20%, 10%, and 0%, respectively. Pathological indicators, including ALT, AST, MDA, GSH, TG, and tissue pathology worsened in line with shortening of the gavage interval and increased gavage dose. Conclusions The alcohol dose, length of the modeling period, and frequency of dosing affect the establishment of an alcoholic liver injury model in mice. We suggest that dosing mice with alcohol with 53% volume ratio twice a day at 20 mL/ kg body weight or 10 mL/ kg body weight at 6 h intervals can successfully cause liver damage.