1.上海中医药大学,上海 200000;2.上海中医药大学附属龙华医院 儿科,上海 200030
1. Shanghai University of Traditional Chinese Medicine, Shanghai 200000, China. 2. Longhua Hospital Shanghai University of Traditional Chinese Medicine, Department of Paediatrics, Shanghai 200030
目的 通过复合过敏性鼻炎和慢性咽炎动物模型建立模拟腺样体肥大大鼠模型,并评价模拟模型的可行性。 方法 通过卵白蛋白腹腔注射基础致敏及滴鼻强化致敏制造过敏性鼻炎大鼠模型,同时予氨水进行大鼠咽喉部喷雾制造慢性咽炎模型,复合成模拟腺样体肥大大鼠模型。造模结束后通过动物行为学评分评估模型动物症状表现;将鼻、咽黏膜及鼻咽淋巴结组织HE 染色后观察其组织病理形态学改变;造模结束取大鼠全血检测嗜酸性粒细胞百分比,ELISA 法检测大鼠血清及粘膜组织IL-4、IgE 水平。 结果 两组模型组动物行为学评分、嗜酸性粒细胞百分比、血清及组织IL-4、IgE 水平均较空白对照组上调(P<0. 01);与模型A 组对比,模型B 组嗜酸性粒细胞百分比、血清及组织IgE 水平上调更加明显(P<0. 01);两组模型组鼻、咽黏膜及鼻咽部淋巴结都出现不同程度的疾病相关组织病理改变。 结论 模拟腺样体肥大大鼠模型在症状表现及病理改变上与腺样体肥大类似,可以通过复合过敏性鼻炎和慢性咽炎动物模型模拟腺样体肥大动物模型,但仍需进一步探索完善。
Objective To evaluate the feasibility of an animal model of simulated adenoid hypertrophy by combining animal models of allergic rhinitis and chronic pharyngitis. Methods A rat model of allergic rhinitis was established by basal sensitization through intraperitoneal OVA injection and nasal enhanced sensitization. A model of chronic pharyngitis was established simultaneously by a throat spray of ammonia, both models were combined into a rat model of simulated adenoid hypertrophy. The symptom performance of model animals was assessed by the animal behavioral score after modeling. Pathomorphological changes of nasal and pharyngeal mucosae and nasopharyngeal lymph nodes were observed by HE staining. The percentage of eosinophils was assess in rat whole blood. IL-4 and IgE levels in rat serum and mucosal tissue were measured by ELISA. Results The behavior score, eosinophil percentage, and IL-4 and IgE levels in serum and tissue were increased compared with the control group (P< 0. 01). Compared with model group A, the eosinophil percentage and serum IgE level were increased in model group B (P<0. 01). In the model groups, nasal and pharyngeal mucosae and nasopharyngeal lymph nodes showed different degrees of disease-related histopathological changes. Conclusions The rat model had symptom manifestations and pathological changes similar to adenoid hypertrophy. This animal model of simulated adenoid hypertrophy can be used as a combined animal model of allergic rhinitis and chronic pharyngitis, but requires further exploration and improvement.
ZHANG Yixing, JIANG Zhiyan, LIU Anqi, WANG Shumin, SUN Chenghui.大鼠腺样体肥大实验动物模型建立的探讨[J].中国比较医学杂志,2023,33(7):9~16.复制